青扦花粉萌发及花粉管生长过程中的钾离子流及BaCl_2和TEA的影响  

Extracellular potassium influx and effect of BaCl_2 and TEA during pollen germination and the growth in Picea wilsonii

作　　者：房克凤[1,2] 张卿 曹庆芹 秦岭 FANG Ke-feng;ZHANG Qing;CAO Qing-qin;QIN Ling(College of Landscape Architecture,Beijing University of Agriculture,Beijing 102206;Beijing Key Laboratory for Agricultural Application and New Technique/College of Plant Science and Technology,Beijing University of Agriculture, Beijing 102206;Beijing Collaborative Innovation Center for Eco Environmental Improvement with Forest and Fruit Trees,Beijing 102206,China)

机构地区：[1]北京农学院园林学院,北京102206 [2]北京林果业生态环境功能提升协同创新中心,北京102206 [3]北京农学院植物科学技术学院/农业应用新技术北京市重点实验室,北京102206

出　　处：《电子显微学报》2017年第5期498-504,共7页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31790719);科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心资助项目(No.2011协同创新)(No.CEFF-PXM2017_014207_000043)

摘　　要：本文以裸子植物青扦花粉为材料,运用非损伤、选择性电极技术探究了花粉水合、萌发及花粉管生长过程中的钾离子流,以及抑制剂处理后的影响。研究结果显示:(1)钾离子通道抑制剂BaCl_2和TEA(氯化四乙胺)处理推迟花粉萌发;(2)BaCl_2和TEA处理后,青扦花粉萌发和花粉管伸长均受到抑制;(3)选择性电极检测结果显示对照花粉不同发育时期钾离子均内流,即花粉水合、萌发及花粉管生长均需大量的钾离子。BaCl_2和TEA处理后,花粉的钾离子流动态也随之发生改变。培养3~9 h,钾离子呈明显的外流,12~21 h钾离子内、外流基本平衡,24 h钾离子内流。抑制剂处理导致的钾离子流的紊乱使得花粉不能正常萌发、花粉管不能正常生长。因而,钾离子在青扦花粉萌发及花粉管生长过程中具有重要的调控作用。Picea wilsonii pollen was chosen as material to study the changes of potassium ions during the first24h of hydration and germination and growth.Two kinds of potassium ion inhibitors of were used to investigate their effects on potassium influx.The results indicated that potassium ion inhibitors(BaCl2and TEA)inhibited the germination and growth of Picea wilsonii pollen.Moreover,the results of scanning ion-selective electrode technique revealed that equilibrium between potassium influx and efflux was detected at3-6h after incubation,and net potassium influx was detected at9to24h of incubation,during which the pollen germinated and the growth.The potassium inhibitors BaCl2and TEA caused potassium efflux at3-9h and an equilibrium of influx and efflux at12-21h,which resulted in the germination of few pollen grains.Thus,potassium ions play a significant role in regulation mechanism of pollen germination and tube growth.

关 键 词：青扦 钾离子 花粉水合 花粉萌发 花粉管生长 

分 类 号：S791.18[农业科学—林木遗传育种]