一种用于标记蓝宝石盘的高压冷冻制样辅助装置  

A sapphire disc marking tool for high pressure freezing sample preparation

作　　者：贾旭东[1] 李红梅[1] 李茵茵[1] 陈东[1] 张勤奋[1] JIA Xu-dong;LI Hong-mei;LI Yin-yin;CHEN dong;ZHANG Qin-fen(School of Life Sciences Sun Yat-Sen University, Guangzhou Guangdong 510275,China;State Key Laboratory for Biocontrol,Sun Yat-Sen University, Guangzhou Guangdong 510275,China)

机构地区：[1]中山大学生命科学学院有害生物控制与资源利用国家重点实验室,广东广州510275

出　　处：《电子显微学报》2017年第5期514-516,共3页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31570736;No.J1310025);NSFC-RGC资助项目(No.31361163001)

摘　　要：高压冷冻技术(high pressure freezing,HPF)是近年来出现的一种可以更有效保存样品原始结构的制样方法。本文介绍了针对高压冷冻制样过程中,无法区分用于培养细胞的蓝宝石盘正反两面的问题,使用3D打印技术制作模具进行喷涂特殊样式的碳膜标记,从而得以区分蓝宝石盘正反面,提高制样成功率。High pressure freezing can efficiently preserve natural cellular ultra-structure.During the sample preparation process,it is hard to distinguish the side with cell of the sapphire disc.To resolve the problem,we developed an auxiliary tool by using a3D printing to lay a carbon film with specific pattern on the sapphire disc,which could mark the right side of sapphire disc.Several results have proved our tool could effectively improve the sample preparation quality

关 键 词：高压冷冻 辅助装置 

分 类 号：Q-337[生物学]