蜂毒肽对成熟树突细胞诱导T细胞分化的免疫调节的影响  

Immune Regulation Influence of Melittin on T Cells Induced Differentiation by Mature Dendritic Cells

作　　者：熊浪平 贺守第 关彤[3] 莫丽华[2] 杨平常[2] 刘志刚[2] 吉琼梅[2] 黄胜光 XIONG Lang ping;HE Shou di;GUAN Tong;MO Li hua;YANG Ping chang;LIU Zhi gang;JI Qiong mei;HUANG Sheng guang(Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China;Institute of Allergy and Immunology,Shenzhen University School of Medicine,Shenzhen 518060,China;The NO.1 Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广州中医药大学,广州510405 [2]深圳大学过敏反应与免疫学研究所,深圳518060 [3]广州中医药大学第一附属医院,广州510405

出　　处：《天然产物研究与开发》2017年第11期1813-1817,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81271950);深圳市科技计划(JCY J20130402151227168;JCYJ20150402152130192;005177);深圳市南山区科技项目(2014046)

摘　　要：通过蜂毒肽(Melittin,MT)干预脂多糖(Lipopolysaccharide,LPS)刺激树突细胞(Dendritic Cell,DC)与淋巴细胞共培养,以探究MT对Th1/Th2分化的影响。用MTS检测LPS、MT对DC与淋巴细胞共培养活性;流式细胞术检测MT对LPS刺激DC与淋巴细胞共培养中Th1、Th2比例;ELISA检测LPS刺激DC与淋巴细胞共培养中细胞上清液中IL-4、INF-γ浓度。结果显示MT、LPS在一定浓度下刺激DC与淋巴细胞共培养的活性;在成熟的DC诱导下,MT能上调Th1/Th2比例、Th1百分率(P<0.05)及IFN-γ浓度(P<0.01);对IL-4浓度、Th2百分率无影响(P>0.05)。本研究说明MT通过上调Th1细胞比例,IFN-γ浓度,促进Th1/Th2细胞向Th1方向分化途径对T细胞起免疫调节作用。To study the intervention of melittin(MT)on dendritic cells(DC)co cultured with lymphocytes stimulated by lipopolysaccharide(LPS),the impact of MT on the differentiation of Th1/Th2was investigated.MTS was used to detect the activity of DC co cultured with lymphocytes by LPS and MT;Flow cytometry was used to detect the ratio of Th1and Th2in the co culture of DCs stimulated by MT and LPS;ELISA was used to detect the concentration IL4,INFγin cell supernatant of MT and LPS stimulated co cultured DC and lymphocyte.The results showed that a certain concentration of MT and LPS can stimulate the co cultured activity of DC with lymphocytes.In the induction of mature DC,MT can increase the Th1/Th2ratio,the Th1percentage(P<0.05)and the IFNγ(P<0.01).But it had no effect on the concentration of IL4and Th2(P>0.05).The study demonstrated that through upregulating the expression of Th1cell ratio and IFNγconcentration,MT promoted the differentiation of Th1/Th2cells to the Th1pathway,thus playing immunomodulatory effects on T cells.

关 键 词：蜂毒肽 树突细胞 T细胞 免疫调节 

分 类 号：R593.22[医药卫生—内科学]