滋肾清肝方对2型糖尿病模型大鼠的药效及作用机制  

Effect and Mechanism of Zishenqinggan Prescription on Type 2 Diabetes Model Rat

作　　者：田春雨 薄海美 曹岩[3] 杨雨旸 喇孝瑾 郭兰 贾永森 李继安 李洁 张碧溦 徐雪梅 TIAN Chun yu;BO Hai mei;CAO Yan;YANG Yu yang;LA Xiao jin;GUO Lan;JIA Yong sen;LI Ji an;LI Jie;ZHANG Bi wei;XU Xue mei(North China University of Science and Technology;Pharmacology Analysis Key Laboratory for Prevention and Treatment of Diabetes of Traditional Chinese Medicine in Hebei Province,Tangshan 063210,China;Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000,China)

机构地区：[1]华北理工大学 [2]河北省中医药防治糖尿病药理分析重点实验室,唐山063210 [3]唐山市中医医院,唐山063000

出　　处：《天然产物研究与开发》2017年第11期1851-1857,共7页Natural Product Research and Development

基　　金：科技部国际科技合作项目(2008DFA31050);河北省中医药管理局科研计划(2016070)

摘　　要：主要研究滋肾清肝方对2型糖尿病模型大鼠的药效及作用机制,为其临床应用及开发奠定基础,将符合2型糖尿病成模标准的大鼠随机分为模型组、二甲双胍组、滋肾清肝方高中低剂量组,给药8周,观察滋肾清肝方对模型大鼠饮食、体重、空腹血糖、糖耐量、糖化血红蛋白、C-肽、胰高血糖素样肽-1、糖化血清蛋白、胰岛素的影响,Western blot测定肝组织AKT、p-AKT、GSK-3β、p-GSK-3β及骨骼肌组织p-IRS-1、IRS-1、Glut4的表达;结果表明滋肾清肝方可调节2型糖尿病模型大鼠糖代谢紊乱,改善胰岛素抵抗,调节骨骼肌PI3K/AKT、肝脏AKT/GSK-3β信号转导通路可能是滋肾清肝方治疗2型糖尿病的作用机制。This study is to investigate the effect and mechanism of Zishenqinggan prescription(ZSQG)on the treatment of type2diabetes,and lay the foundation for its clinical application and further development.T2DM model rats were randomly divided into model group,metformin group,high,middle,low dose of ZSQG group.The effect of ZSQG on body weight,food intake,water intake,urine,stool,blood glucose,OGTT,HbAlc,C peptide,GLP1content,GSP,insulin of rats was studied;Western blot was used to detect AKT,p AKT,GSK3β,p GSK3βexpression in liver tissue and to detect p IRS1,IRS1,Glut4expression in skeletal muscle tissue.ZSQG can effectively regulate sugar metabolic disorders in type2diabetic model rat,improve insulin resistance;enhance insulin secretion,regulate skeletal muscle PI3K/AKT,regulate liver AKT/GSK3βsignaling pathway,which may be the mechanism of ZSQG for the treatment of type2diabetes and to improve insulin resistance.

关 键 词：滋肾清肝方 2型糖尿病 药效 作用机制 

分 类 号：R285.5[医药卫生—中药学]