紫红獐牙菜中的酮类成分及其细胞毒活性  

Xanthones from Swertia punicea and Their Cytotoxic Activity

作　　者：雷声 郭青 王明锋 刘文星[2] 牟林云 李干鹏[2] LEI Sheng;GUO Qing;WANG Ming feng;LIU Wen xing;MOU Lin yun;LI Gan peng(Technology center of China tobacco Yunnan industrial company Limited,Kunming 650231,China;Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission & Ministry of Education,Yunnan Minzu University,Kunming,650500,China)

机构地区：[1]云南中烟工业有限责任公司技术中心,昆明650231 [2]云南民族大学民族药资源化学国家民委教育部重点实验室,昆明650500

出　　处：《天然产物研究与开发》2017年第11期1877-1881,共5页Natural Product Research and Development

基　　金：云南中烟工业有限责任公司,烟草行业卷烟调香技术重点实验室科研项目(JSZX20151008-52)

摘　　要：采用色谱分离手段对紫红獐牙菜Swertia punicea的乙醇提取物中乙酸乙酯和石油醚萃取部分进行分离纯化,运用1H NMR、13C NMR、HSQC、HMBC和ESI-MS等波谱方法和文献数据对比鉴定化合物结构,从紫红獐牙菜全草中得到8个口山酮类化合物,分别鉴定为1-羟基-2,3,5,7-四甲氧基口山酮(1)、1,2,3,4,5-五甲氧基口山酮(2)、1-羟基-2,3,4,7-四甲氧基口山酮(3)、1-羟基-2,3,5-三甲氧基口山酮(4)、dulcisxanthone C(5)、1-羟基-2,3,7-三甲氧基口山酮(6)、1,5-二羟基-2,3-二甲氧基口山酮(7)、1-羟基-3,4,5-三甲氧基口山酮(8),所有化合物均为首次从该植物中分离得到,化合物1、4、5、7对Hep G-2显现了很好的细胞毒活性。The xanthones from Swertia punicea and their cytotoxic activities were investigated in this study.The petroleum ether and ethyl acetate part of ethanol extracts from S.punicea were separated by chromatographic techniques.The structures of these compounds were identified by1H NMR,13C NMR,HSQC,HMBC and ESI MS data,eight xanthones were isolated from this plant,they were1hydroxyl2,3,5,7tetramethoxyxanthone(1),1,2,3,4,5pentamethoxythone(2),1hydroxyl2,3,4,7tetramethoxyxanthone(3),1hydroxyl2,3,5trimethoxyxanthone(4),dulcisxanthone C(5),1hydroxyl2,3,7trimethoxyxanthone(6),1,5dihydroxy2,3dimethoxyxanthone(7)and1hydroxyl3,4,5trimethoxyxanthone(8),all of these compounds were isolated for the first time from S.punicea.The cytotoxic activities of these compounds were also evaluated,and compound1,4,5,7showed good cytotoxic activities against HepG2.

关 键 词：紫红獐牙菜 口山酮 细胞毒活性 二维核磁共振 

分 类 号：R284.2[医药卫生—中药学]