8株草本科植物根际土放线菌代谢产物活性初步研究  

Preliminary Study on the Activities of Metabolites of Rhizosphere Soil Actinomycetes from Eight Herbaceae Plants

作　　者：车敏娜[1] 吴恒 热孜万古力.赛买提 王国威 郑棱峻 王胜宝 陈士恩[1] CHE Min na;WU Heng;RE Ziwanguli·Saimaiti;WANG Guo wei;ZHENG Leng jun;WANG Sheng bao;CHEN Shi en(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730124,China)

机构地区：[1]西北民族大学生命科学与工程学院,兰州730124

出　　处：《天然产物研究与开发》2017年第11期1920-1927,共8页Natural Product Research and Development

基　　金：因家民委专项综合改革试点(10019141);国家科技支撑计划(2015BAD29B05);西北民族大学中央专项资金资助研究生科研创新项目(Yxm2017121)

摘　　要：为了探讨温度对西北民族大学榆中校区8株草本科植物根际放线菌出菌率的影响以及对放线菌代谢产物活性的影响进行初步研究,为后续放线菌筛选以及培养最适温度方面提供基础资料。试验采用含有75μg/m L重铬酸钾和2μg/m L青霉素的甘油精氨酸琼脂培养基,对菌种进行23、60、80、100及120℃五个不同温度的预处理,利用稀释平板涂布法对8种草本科植物根系土壤样品进行分离,初步探讨温度对放线菌数量和种类的影响;利用7种筛选培养基对所得菌株定性检测,进一步探讨功能酶产生菌的生理生化特性。结果表明:通过23、60、80、100及120℃五种不同温度试验,同一土样在不同温度预处理下放线菌的数量和种类差异显著;发现18株放线菌中有9株放线菌产淀粉酶,16株产蛋白酶,16株产脲酶,18株产过氧化氢酶,12株产聚氧乙烯失水山梨醇单月桂酸酯酶,14株产聚氧乙烯失水山梨醇脂肪酸酯酶,7株产聚氧乙烯山梨醇酐单油酸酯酶,5株产色氨酸酶,5株产纤维素酶;不同温度筛选的放线菌产酶种类不尽相同。说明西北民族大学榆中校区草本科植物根际放线菌产酶特性良好,是淀粉酶、蛋白酶、酯酶等酶的重要来源;随着预处理温度升高,放线菌数量均随着温度升高呈降低趋势;同一土样在不同温度下放线菌的种类与温度没有相关性,但是特定的温度会筛选特定的菌株,即某些菌对温度有一定的选择性。The aim of this study was to investigate the effects of temperature on the rate of rhizosphere actinomycetes and the activity of actinomycetes in eight Chinese herbal medicine plants in Yuzhong campus of Northwest Minzu University,and to provide a preliminary study on the follow up actinomycete screening.The glycerol arginine agar medium containing75μg/mL potassium dichromate and2μg/mL penicillin was used to pretreat the five different temperatures of23,60,80,100and120℃.The effects of temperature on the number and species of actinomycetes were studied by plate coating method.The results showed that the effects of temperature on the number and species of actinomycetes were qualitatively detected by seven kinds of screening medium,and the physiology of functional enzyme producing bacteria was further explored by biochemical testing.The results showed that there were9strains of actinomycetes in18strains of actinomycetes,and the number and species of actinomycetes were different in different soils at different temperatures by23,60,80,100and120℃.Amylase production,16strains of protease,16strains of urease,18strains of catalase,12strains of polyoxyethylene sorbitan monolaurate,14strains of polyoxyethylene sorbitan fatty acid Esterase,7strains of polyoxyethylene sorbitan monooleate,5strains of tryptophanase and5cellulases.The different types of actinomycetes were different.The results showed that the activities of amylase,protease and esterase were an important source of enzyme,and the number of actinomycetes increased with the increasing of pretreatment temperature.The species of actinomycetes at different temperatures had no correlation with temperature,but the specific temperature will be used to screen specific bacteria,that is,some bacteria have a certain selectivity to temperature.

关 键 词：草本科植物 根际土 放线菌 代谢产物 

分 类 号：Q93-335[生物学—微生物学]