三种绿原酸提取物的抑菌和抗氧化效果比较  

Antimicrobial and Antioxidant Effects of 3 Chlorogenic Acid Extracts

作　　者：胡居吾[1] 韩晓丹[1] 付建平[1] 王慧宾[1] 李雄辉[1] HU Ju wu;HAN Xiao dan;FU Jian ping;WANG Hui bin;LI Xiong hui(Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330029,China)

机构地区：[1]江西省科学院应用化学研究所,南昌330096

出　　处：《天然产物研究与开发》2017年第11期1928-1933,共6页Natural Product Research and Development

基　　金：江西省重点科技支撑项目(20171ACF60009);江西省科学院重点项目(2016034)

摘　　要：本文研究平卧菊三七、金银花、杜仲叶绿原酸提取物的抑菌和抗氧化作用,为选择合适的天然食品防腐剂和抗氧化剂提供参考。采用纸片扩散法,探讨平卧菊三七、金银花、杜仲叶绿原酸提取物分别对几种常见致病菌的抑菌活性。在抗氧化作用方面,探讨平卧菊三七、金银花、杜仲叶绿原酸提取物分别在抗脂质过氧化能力、还原能力和清除DPPH·方面进行了研究。结果表明,三种植物绿原酸提取物对细菌均有很强的抑制作用,特别是对金黄色葡萄球菌的抑制作用,在浓度为100 mg/m L时,平卧菊三七绿原酸提取物、金银花绿原酸提取物、杜仲叶绿原酸提取物的抑菌圈分别可达21.4、23.6、24.7 mm。同时,对大肠杆菌和沙门氏菌液也有明显的抑制作用,但是对酵母菌的抑菌作用不明显;且各供试物的抑菌强弱顺序为:杜仲叶绿原酸提取物>金银花绿原酸提取物>平卧菊三七绿原酸提取物。三种植物中绿原酸提取物中,杜仲叶绿原酸提取物的抗脂质过氧化能力、还原能力和清除DPPH·能力均高于其他两种绿原酸提取物。本文揭示了在平卧菊三七、金银花、杜仲叶这三种绿原酸提取物中,杜仲叶绿原酸提取物有较强的抑菌活性和抗氧化作用。The aim of this study was to study antimicrobial and antioxidant effects of chlorogenic acid extracts from Gynuraprocumbens,Lonicera japonicaThunb.and Eucommiaulmoides leaves,and to provide a reference for its usage as a natural food preservative and antioxidant.The antibacterial effects were studied using disc diffusion method against several common pathogens.The antioxidant effect of chlorogenic acid extractsfrom G.procumbens,L.japonica and E.ulmoides leaves,including anti lipid peroxidation,reducing capacity,DPPH scavenging ability were studied respectively.The inhibition of chlorogenic acid extracts on bacteria were very strong,especially on Staphylococcus aureus,at the concentration of100mg/mL,the bacteriostatic ring were up to21.4mm,23.6mm,24.7mm,respectively for chlorogenic acidextracts from the three species.At the same time,it had obvious inhibitory effects on E.coli and S.bacteria,but the inhibitory effect on the yeast was not obvious.The strength and the sequence of antibacterial compounds were:L.japonicachlorogenic acid extract,E.ulmoideschlorogenic acid extract,G.procumbenschlorogenic acid extract.The anti lipid peroxidation ability,reducing power and DPPH scavenging ability of L.japonicachlorogenic acidextract were higher than those of the other two extracts.L.japonicachlorogenic acid extract had a stronger antibacterial activity and antioxidant effect among three chlorogenic acidextracts.

关 键 词：绿原酸提取物 抑菌效果 抗氧化作用 

分 类 号：S38[农业科学—农艺学]