川芎油对氯化钴损伤PC12细胞保护作用及其活性成分研究  

Protective Effects of Ligusticum chuanxiong Oil on PC12 Cells Injured by CoCl_2 and Its Active Components

作　　者：王月[1] 刘露丝 刘娟[1] 彭成[1] 熊亮[1] WANG Yue;LIU Lu si;LIU Juan;PENG Cheng;XIONG Liang(State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《天然产物研究与开发》2017年第11期1940-1945,共6页Natural Product Research and Development

基　　金：四川省科技支撑计划川芎整合式全产业链综合开发研究(2015SZ0031);科技基础性工作专项计划(2015FY111500-140);四川省青年科技创新研究团队专项计划(2017TD0001)

摘　　要：综合运用硅胶、Sephadex LH-20柱色谱和半制备高效液相色谱等方法对川芎挥发油进行分离纯化,根据理化性质及波谱数据鉴定化合物结构。利用氯化钴诱导PC12细胞建立神经损伤模型,通过MTT法、Hoechst染色法及乳酸脱氢酶试剂盒测定川芎及其单体成分对损伤神经细胞的保护作用。结果显示:从川芎挥发油中分离得到6个化合物,分别鉴定为riligustilide(1)、endo-Z,Z'-(3a.7a',7a.3a')-双藁本内酯(2)、匙叶桉油烯醇(3)、(E)-丁烯基苯酞(4)、7-羟基-丁烯基苯酞(5)和洋川芎内酯I(6)。发现川芎挥发油及化合物1显著提高氯化钴损伤的PC12细胞的存活率,降低细胞内乳酸脱氢酶水平,抑制PC12细胞凋亡。表明川芎挥发油及化合物1在一定浓度范围内具有保护神经细胞的作用,为进一步阐述川芎挥发油的药效物质基础提供科学依据。The chemical constituents of the essential oil from Ligusticum chuanxiong were separated and purified by various chromatographic techniques including silica gel and Sephadex LH20column chromatography,normal phase(NP)semi preparative HPLC and reversed phase(RP)semi preparative HPLC.Their structures were identified by physicochemical properties,spectroscopic analysis,as well as comparison with the literature data.PC12cells injured by CoCl2were used as the cell model of hypoxia.The viability and lactate dehydrogenase(LDH)release of PC12cells were separately detected by MTT assays and LDH detecting kits,and the apoptotic cells were stained by Hoechst33258.Six compounds were isolated from the essential oil of L.chuanxiong and identified asriligustilide(1),endo Z,Z′(3a.7a′,7a.3a′)diligustilide(2),spathulenol(3),(E)3butylidenephthalide(4),3butylidene7hydroxyphthalide(5),and senkyunolide I(6).It was proved that the oil and compound1(riligustilide)protected the injury and inhibit the apoptosis induced by CoCl2in PC12cells,and reduced the level of LDH in cells.These results demonstrated that the essential oil of L.chuanxiong and compound1(riligustilide)had protective effect for injured neurons in appropriate concentration range,and provided scientific reference for pharmacodynamic material basis of L.chuanxiong.

关 键 词：川芎 挥发油 化学成分 PC12细胞保护 

分 类 号：R285.5[医药卫生—中药学]