一株耐锌菌株的分离鉴定及其生长特性研究  

Isolation and identification of a zinc-resistance strain and research on its growth characteristics

作　　者：曹礼[1] 李晓慧 张芹辉 贾东波 孙晓杰 徐丽梅 雷玉明[1] CAO Li;LI Xiao hui;ZHANG Qin hui;JIA Dong bo;SUN Xiao jie;XU Li mei;LEI Yu ming(College of Agriculture and Biotechnology; Key Laboratory of Hexi Corridor Resourses Utilization of Gansu, Hexi University, Zhangye 734000, China)

机构地区：[1]河西学院农业与生物技术学院甘肃省河西走廊特色资源利用重点实验室,张掖734000

出　　处：《生物学杂志》2017年第6期60-64,共5页Journal of Biology

基　　金：河西走廊特色资源利用重点实验室项目(No.XZ1401)

摘　　要：从造纸厂排污口的土壤中分离对锌具有较强耐受性的菌株HXZ-1。菌株HXZ-1能够在含锌离子浓度为50 mg/L的液体培养基中生长良好,能耐受锌离子的最高浓度为1000 mg/L。结合其生理生化特征和16S rRNA基因系统发育分析,将该菌株鉴定为原小单孢菌属(Promicromonospora sp.HXZ-1)。菌株HXZ-1最适培养条件为:锌离子浓度为50 mg/L、葡萄糖为碳源、蛋白胨为氮源。当锌离子浓度为50 mg/L时,菌株HXZ-1对锌吸附率最大,为29.1%;当锌离子浓度为500 mg/L时,培养时间为10 h时,菌株HXZ-1吸附率最大,为18.8%。菌株HXZ-1是一株对锌有较强抗性和吸附性的细菌,为重金属污染的微生物修复提供参考。A bacterium with strong tolerance to zinc,strain HXZ1,was isolated from sewage of a paper mill.It could tolerate1000mg/L of zinc ion.Based on the phylogenetic analysis of the16S rRNA gene and the physiologic&biochemical characteristics,it was identified as Promicromonospora sp.HXZ1.The culture conditions of strain HXZ1were zinc ion concentration of50mg/L,glucose as carbon source and peptone as the nitrogen source.When the concentration of zinc ions in cultural liquid was50mg/L,the zincic adsorption rate of HXZ-1reached the maximum of291%;the maximum zincic adsorption rate of strain HXZ1run up to188%after cultured10h in concentration of zinc ions as500mg/L.Strain HXZ1had better adsorption effect and tolerance to zinc.

关 键 词：耐锌 16S-rRNA 原小单孢菌属 生长特性 

分 类 号：Q93-331[生物学—微生物学] Q935