生物组织光学透明技术研究进展  

The research progress of tissue optical clearing techniques

作　　者：李亚敏 薛成志[1,2,4] 李贵叶 胡章立 阮双琛 陈玲玲 LI Ya min;XUE Cheng zhi;LI Gui ye;HU Zhang li;RUAN Shuang chen;CHEN Ling ling(Shenzhen Key Laboratory of Laser Engineering;Key Laboratory of Advanced Optical Precision Manufacturing Technology of Guangdong Higher Education Institutes, Shenzhen University;Shenzhen University, College of Optoelectronic Engineering, Shenzhen 518060;College of Electronic Science and Technology, Shenzhen University, Shenzhen 518060;College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China)

机构地区：[1]深圳市激光工程重点实验室 [2]深圳大学先进光学精密制造技术广东普通高校重点实验室 [3]深圳大学生命与海洋科学学院,深圳518060 [4]深圳大学电子科学与技术学院,深圳518060 [5]深圳大学光电工程学院电子科学与技术学院生命与海洋科学学院,深圳518060

出　　处：《生物学杂志》2017年第6期83-88,共6页Journal of Biology

基　　金：国家自然科学基金(No.61505112);中央高校基本科研业务费专项资金资助;深圳市战略新兴产业发展专项资金资助(No.JCYJ20150525092941015);深圳市高端人才启动(827-0000086)

摘　　要：生物组织光学透明技术是一种将完整的不透明的生物组织器官经过光学透明处理后实现高透明且完整的新技术,可进一步结合光学成像技术实现完整组织器官的荧光/吸收三维立体成像,具有传统切片技术无法替代的性能,是生物医学光子学领域的研究热点。综述了组织光学透明技术的概念、原理及具体的实现方法和步骤,介绍了其在生物医学尤其是脑科学领域的研究现状和部分成果,比较了各方法不同参数的优劣,并就未来的挑战和发展应用研究进行了讨论。Tissue optical clearing methods,which transform intact opaque tissue into an optically transparent construct by reducing light scattering and then can be further combined with optical imaging technology to facilitate intact tissue organ deep optical(fluorescence/absorption)three dimensional imaging,have been attracted increasing interest in biomedical photonics area due to their advantages compared to conventional mechanical sectioning based methods.This paper summarized optical clearing principles,main approaches and their current research situation and achievements in biomedicine,especially in the field of brain science.The comparison of their performance parameters and the further development challenge were also presented.

关 键 词：生物组织光学透明 组织透明 生物系统样本成像 生物医学光子学 

分 类 号：Q593[生物学—生物化学]