基于金属纳米粒子增敏的SPR生物传感器检测吲哚乙酸  

Determination of Indole Acetic Acid by SPR Biosensor Based on the Metal Nanoparticles Sensitization

作　　者：高文慧 王兆群[1] 泮俊青 尹洪宗[1] GAO Wenhui;WANG Zhaoqun;PAN Junqing;YIN Hongzong(College of Chemistry and Material Science ,Shandong Agricultural University,Taian 201718, Shandong, P. R. China)

机构地区：[1]山东农业大学化学与材料科学学院,山东泰安271018

出　　处：《影像科学与光化学》2017年第6期843-852,共10页Imaging Science and Photochemistry

基　　金：国家自然科学基金项目(31171953)资助

摘　　要：本实验建立了表面等离子体共振(SPR)生物传感器检测3-吲哚乙酸(IAA)的方法。制备了两种SPR生物传感器检测IAA:传统模式的SPR生物传感器1和Au/Ag合金纳米粒子增敏的SPR生物传感器2。结果发现:传感器1在IAA浓度范围为175~350μg/L时,浓度与其波数位移值呈线性关系,检出限为25μg/L(S/N=3);传感器2在IAA浓度范围为17.5~250μg/L时,浓度与其波数位移值呈线性关系,检出限为2.2μg/L(S/N=3)。说明基于Au/Ag合金纳米粒子的传感器2比传感器1有较高的灵敏度和较低的检出限。加标回收实验测得加标回收率范围为96%~100.2%,平均值为98.4%。本实验制备的SPR生物传感器具有较好的精密度、稳定性、重现性和特异性。Surface plasmon resonance(SP R)biosensor was a new method for detecting3-indole acetic acid(IA A).We adopted two kinds of indole acetic acid SPR biosensors to detect IA A:Traditional sensor1and A u/Ag alloy nanoparticles based sensor2.Sensor1:The wavenumber shifts were linearly proportional to the IAA concentration in a range from175to350jitg/L with a detection limit of25jitg/L(S/N=3).Sensor2:The wavenumber shifts were linearly proportional to the IAA concentration in a range from17.5to250jitg/L with a detection limit of2.2jitg/L(S/N=3).The results showed that the sensor2based on A u/A g alloy nanoparticles had higher sensitivity and lower detection limit than the the sensor1.The rate of standard addition recovery was96%-!00.2%,the average was98.4%.The prepared SPR biosensor had good precision,stability,repeatability and specificity.

关 键 词：表面等离子体共振生物传感器 吲哚乙酸 吲哚乙酸抗体 Au/Ag合金纳米粒子 

分 类 号：O657.3[理学—分析化学] TP212.3[理学—化学]