北五味子多糖对高脂诱导非酒精性脂肪性肝病大鼠的降血脂作用及其抗氧化活性  

Lipid-lowering effect and antioxidant activity of polysaccharide from Schisandra Chinensis in rats with non-alcoholic fatty liver disease induced by high-fat diet

作　　者：苑荣爽 李贺[1] 孙靖辉[1] 庄文越[2] 陈建光[1] 王春梅[1] YUAN Rongshuang;LI He;SUN Jinghui;ZHUANG Wenyue;CHEN Jianguang;WANG Chunmei(Department of Pharmacology, College of Pharmacy, Beihua University, Jilin 132013, China;Department of Molecular Biology, College of Laboratory Medicine, Beihua University, Jilin 132013, China)

机构地区：[1]北华大学药学院药理学教研室,吉林吉林132013 [2]北华大学医学检验学院分子生物学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2017年第6期1103-1108,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅医药产业推进计划项目资助课题(20140311052YY);吉林省科技厅产业技术创新战略联盟项目资助课题(20150309004YY)

摘　　要：目的:探讨北五味子多糖(SCP)对高脂饮食诱导非酒精性脂肪性肝病(NAFLD)大鼠的降血脂作用及其抗氧化活性,为五味子的开发利用提供理论依据。方法:选择32只雄性Wistar大鼠,随机抽取16只作为正常对照组(灌胃给予双蒸水,给予普通饲料)和SCP组(灌胃给予50mg·kg-1 SCP,给予普通饲料),每组8只。其余16只大鼠以高脂饲料喂养4周,确定高脂血症成功后,将大鼠随机分为NAFLD组和NAFLD+SCP组(NAFLD+50mg·kg-1 SCP),给药12周后称大鼠体质量并计算肝指数;检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)和门冬氨酸氨基转移酶(AST)水平,酶学法检测大鼠肝组织中TC和TG水平,TBA法检测大鼠血清和肝组织中丙二醛(MDA)水平,黄嘌呤氧化酶法检测超氧化物歧化酶(SOD)活性,微量酶法检测肝组织中还原型谷胱甘肽(GSH)水平,HE染色观察大鼠肝组织病理形态表现。结果:与正常对照组比较,NAFLD组大鼠肝指数明显增加(P<0.01),血清中TC、TG、LDL-C、ALT和AST水平升高(P<0.01),肝组织中TG和TC水平升高(P<0.01),血清及肝组织中MDA水平升高(P<0.01)、SOD活性降低(P<0.01),肝组织中GSH水平降低(P<0.01)。与NAFLD组比较,NAFLD+SCP组大鼠体质量及肝脂数明显降低(P<0.05),血清中TC、TG及LDL-C水平以及肝组织中TC和TG水平降低(P<0.01),血清及肝组织中MDA水平降低(P<0.01),SOD活性升高(P<0.01),肝组织中GSH水平升高(P<0.01)。HE染色检测,NAFLD组大鼠肝小叶结构紊乱,出现明显的肝细胞脂肪变性;NAFLD+SCP组大鼠肝小叶内肝细胞脂肪变性明显减轻。结论:SCP对高脂饮食诱导的NAFLD大鼠血脂具有一定的调节作用,其作用机制可能与抗氧化应激有关。Objective:To investigate the lipid lowering effect and antioxidant activity of polysaccharide from Schisandra Chinensis(SCP)in the rats with non alcoholic fatty liver disease(NAFLD)induced by high fat diet,and to provide a theoretical basis for the development and utilization of Schisandra Chinensis.Methods:A total of32male Wistar rats were selected.Sixteen from the32rats were randomly selected and divided into normal control group(intragastrical administration of water,combined with normal diet,n=8)and SCP group(intragastrical administration of50mg·kg-1SCP,combined with normal diet,n=8).The remaining16rats were fed with high fat diet for4weeks and the confirmed NAFLD rat models were set up.A total of16NAFLD rats were randomly divided into NAFLD group(intragastrical administration of water,combined with high fat diet,n=8)and NAFLD+SCP group(intragastrical administration of50mg·kg-1SCP,combined with high fat diet,n=8).After treated for12weeks,the body weights of all the rats were weighed and the liver index was calculated.The levels of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL C),high density lipoprotein cholesterol(HDL C),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum of all the rats were determined.The levels of TC and TG in liver tissue of the rats were measured by enzymatic method.The malondialdehyde(MDA)levels and superoxide dismutase(SOD)activities in serum and liver tissue and the glutathione(GSH)levels in liver tissue of the rats were analyzed by TBA,xanthinoxidase and microscale enzyme methods,respectively.HE staining was used to observe the pathomorphology of liver tissue of the rats.Results:Compared with normal control group,the liver index of the rats in NAFLD group was increased(P<0.01);the levels of TC,TG,LDL C,ALT and AST in serum of the rats were increased(P<0.01),the levels of TC and TG in liver tissue of the rats were increased(P<0.01),the MDA level was increased(P<0.01)and the SOD activity was decreased(P<0.01),and the GSH

关 键 词：北五味子 多糖 非酒精性脂肪性肝病 氧化应激 

分 类 号：R285.5[医药卫生—中药学]