柴胡多糖硫酸化修饰条件的优化及其修饰前后抗氧化能力比较  

Optimization of sulfated modification conditions of polysaccharide fromBupleurum Chinense and comparison of their antioxidant abilities between before and after modification

作　　者：宋见喜[1] 任婷 满枋霖 冯丽娟[1] 孙新[2] 佟海滨[2,3] SONG Jianxi;REN Ting;MAN Fanglin;FENG Lijuan;SUN Xin;TONG Haibin(Analtical and Testing Center, Beihua University, Jilin 132013, China;Life Science Research Center,Jilin Provincial Key Laboratory of Molecular Geriatric Medicine, Beihua University, Jilin 132013, China;Resource Center for Chinese Materia Medica, Center for Post Doctoral Research, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]北华大学分析测试中心,吉林吉林132013 [2]北华大学生命科学中心吉林省分子老年医学重点实验室,吉林吉林132013 [3]中国中医科学院博士后科研流动站中药资源中心,北京100700

出　　处：《吉林大学学报（医学版）》2017年第6期1165-1170,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(31401203);中国博士后科学基金资助课题(2017M610155);吉林省教委科研基金资助课题(吉教科合字[2016]第53号)

摘　　要：目的:探讨硫酸化修饰柴胡多糖(BCP)的影响因素,阐明硫酸化柴胡多糖(S-BCP)抗氧化能力提升的可能机制。方法:利用氯磺酸-吡啶法对BCP进行了硫酸化修饰。通过调节氯磺酸与吡啶的体积比(1∶2、1∶4和1∶8),观察S-BCP的取代度(DS);应用红外(IR)光谱初步分析BCP和S-BCP的结构,扫描电子显微镜(SEM)下观察分析BCP和S-BCP的表面形态表现。基于1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力建立抗氧化模型,实验分为阳性对照组、BCP组和S-BCP组,检测各组DPPH自由基的清除率。结果:当氯磺酸与吡啶的体积比为1∶4、反应时间为2h、反应温度为60℃时,制得S-BCP的含硫百分率最大值为18.62%,DS最大值为2.32。与BCP组比较,各剂量S-BCP组DPPH自由基的清除率升高(P<0.05)。结论:氯磺酸与吡啶的体积比影响S-BCP的DS值,BCP经硫酸化修饰后通过改变多糖的理化性质和空间构象而提升其抗氧化能力。Objective:To investigate the influencing factors of sulfated modification of Bupleurum chinense polysaccharides(BCP),and to elucidate the possible mechanism of improving the antioxidant ability of sulfated BCP(S BCP).Methods:BCP was sulfated by chlorosulfonic acid pyridine method.The degree of substitution(DS)of S BCP was observed by adjusting the volume ratios of chlorosulfonic acid to pyridine(1∶2,1∶4,and1∶8).The structures of BCP and S BCP were analyzed by infrared(IR)spectroscopy,the morphology of BCP and S BCP were observed under scanning electron microscopy(SEM).The antioxidant model was established by using1,1diphenyl2picrylhydrazyl(DPPH)free radical scavenging.The experiment was divided into positive control group,BCP group and S BCP group,and the scavenging rates of DPPH free radical in various groups were.compared.Results:When the volume ratio of chlorosulfonic acid to pyridine was1∶4,the reaction time was2h and the reaction temperature was60℃,the maximum sulfur content percentage of S BCP was1862%and the DS was the highest(DS=232)..compared with BCP group,the scavenging rate of DPPH free radical of S BCP was significantly increased(P<0.05).Conclusion:The volume ratio of chlorosulfonic acid to pyridine can affect the DS of S BCP.The sulfated modification can increase the anti oxidant capacity of BCP by changing its physic chemical characters and spatial conformation.

关 键 词：柴胡 多糖 硫酸化 抗氧化 

分 类 号：Q539[生物学—生物化学]