STK15 mRNA、STAT3-siRNA对胃癌细胞株表达实验研究  

Expression of STK15 mRNA and STAT3-siRNA in gastric carcinoma cell line

作　　者：孟玮[1] 史晓宇[1] 孙守燕[1] 张敬[1] 宗治国[2] 王世旭[1] 马峰[1] 赵峻峰[1] Meng Wei;Shi Xiaoyu;Sun Shouyan(Department of Medical Oncology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000)

机构地区：[1]河北北方学院附属第一医院肿瘤内科,张家口075000 [2]河北北方学院附属第一医院骨外科,张家口075000

出　　处：《陕西医学杂志》2017年第11期1494-1497,共4页Shaanxi Medical Journal

基　　金：河北省卫生和计划生育委员会医学科学研究重点课题(20160361)

摘　　要：目的:研究中心体扩增激酶STK15以及信号传导与转录激活因子3(STAT3)小干扰RNA(siRNA)在胃癌中的表达。方法:采用Real-time荧光定量PCR技术对7例胃癌新鲜组织和2例正常胃粘膜组织中STK15的mRNA表达水平进行检测。采用体外转染实验以及荷瘤裸鼠体内实验评价STAT3-siRNA的体内外抗肿瘤效果。结果:STK15在胃癌组织的表达量明显高于正常胃黏膜组织,差异有统计学意义(P<0.01)。另外,STAT3-siRNA能够显著降低胃癌细胞的体外增殖能力。结论:中心体扩增是细胞癌变过程的重要事件,中心体的异常扩增是细胞恶性克隆的关键环节。STAT3-siRNA显著降低胃癌细胞的体内外增殖能力和克隆形成能力。Objective:To study the expression of centrosome amplification kinase STK15and transcription factor3(S T A T3)small interfering RNA(siR N A)in gastric carcinoma.Methods:The expression of STK15mRN A in gastric tissue was detected by Real-time fluorescence quantitative PCR.In vitro and in vivo antitumor effects of STAT3-siRN A were evaluated by in vitro transfection experiments and tumor-bearing nude mice.Results:The expression of STK15in gastric cancer was1.326dz0.072,0.655dz0.053in normal gastric tissue,the difference was significant(P〈0.01).STAT3-siRN A significantly reduced the ability of gastric cancer cells to proliferate and clone formation ability in vitro.Conclusion:Central body amplification is an important event in the process of cell carcinogenesis.Abnormal amplification of central body is the key link of malignant cell cloning.STAT3-siRN A significantly reduced the ability of gastric cancer cells in vitro and in vivo and tumorigenic ability.Which may play a role by inhibiting the expression of PCNA.

关 键 词：胃肿瘤/病理学 转录激活因子3/分析 @丝氨酸/苏氨酸激酶15 基因扩增 中心体 

分 类 号：R392.3[医药卫生—免疫学]