不同浓度荨麻提取物对骨关节炎作用的差异  

Anti-osteoarthritis activity of urtica extracts at different concentrations

作　　者：郑晓芬 Zheng Xiao-fen(Nurse-in-charge, Longhua People’s Hospital in Shenzhen, Shenzhen 518109, Guangdong Province, China)

机构地区：[1]深圳市龙华区人民医院,广东省深圳市518109

出　　处：《中国组织工程研究》2017年第36期5741-5746,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:荨麻作为一种传统中药,有抗炎、消肿和抗风湿作用,其对骨关节炎的效果目前尚未见相关研究。目的:探讨不同浓度荨麻对骨关节炎的治疗效果及其机制。方法:(1)取18只大鼠剔除表面部分毛,行皮肤刺激实验,分为液体石蜡组、荨麻组和甲醛组,每组6只,分别在大鼠皮肤上面涂石蜡、荨麻胶体和0.8%的甲醛,观察皮肤皮疹和红肿情况;(2)取30只大鼠,使用木瓜蛋白酶诱导法建立大鼠骨关节炎模型,根据荨麻提取物的浓度,实验分为100 mg/kg组、200 mg/kg组、400 mg/kg组和阳性对照组,每组6只,给药1,2,3周分别测定大鼠左膝关节直径,判断膝关节肿胀情况;用0.6%的醋酸溶液建立大鼠急性疼痛模型,分别给予不同浓度荨麻,对照组给予生理盐水;(3)对比观察大鼠翻滚及扭转次数,判断荨麻对大鼠止痛效果;给药后第1,2,3周分别用Elisa法测大鼠左膝关节的关节液中白细胞介素1β、肿瘤坏死因子α和基质金属蛋白酶3的含量,检测大鼠关节炎症因子情况。结果与结论:(1)荨麻作用于大鼠皮肤后,皮肤表面未见水肿及红疹;(2)质量浓度为200 mg/kg及400 mg/kg的荨麻与对照组相比能减少关节肿胀、其对关节肿胀的减轻作用和吲哚美辛相比无明显差别;(3)质量浓度200 mg/kg及400 mg/kg的荨麻能减轻大鼠痛疼,其减轻疼痛效果和吲哚美辛相当;(4)荨麻在质量浓度为200 mg/kg及400 mg/kg时,与对照组相比能减大少鼠关节液中炎症因子白细胞介素1β、肿瘤坏死因子α和基质金属蛋白酶3的含量;(5)结果表明,荨麻具有抗炎、止痛、消肿和抗骨关节炎的作用。BACKGROUND:Urtica,as a traditional Chinese medicine,is famous for anti-inflammatory,detumescence and anti-rheumatic effects,but its effect on osteoarthritis remains unknown.OBJECTIVE:To investigate the therapeutic effect of different concentrations of urtica on osteoarthritis and the underlying mechanism.METHODS:(1)Eighteen rats were selected,and after their hair was shaved,the rats were divided into three groups(n=6per group).The rat skin was painted with paraffin,urtica colloid and0.8%formaldehyde,respectively,and the skin rash and swelling were observed.(2)Thirty rats were selected,and the rat model of osteoarthritis was established by injecting papain,followed by equally allotted into100,200,and400mg/kg urtica and positive control groups.The diameter of the rat left knee was measured at1,2and3weeks after administration to determine the severity of knee swelling.The rat model of acute pain was established with0.6%acetic acid solution,and then the model rats were subjected to different concentrations of urtica or normal saline(control group).(3)The frequency of rat tumbling and twisting was observed to determine the analgesic effect of urtica.The levels of interleukin-1β,tumor necrosis factor-αand matrix metalloproteinase3in the synovial fluid of the left knee joint were detected by ELISA at1,2and3weeks after administration.RESULTS AND CONCLUSION:After urtica administration,no edema or rash appeared on the rat skin surface.Compared with the control group,200and400mg/kg urtica could reduce joint swelling and pain,showing no significant difference from indomethacin.Compared with the control group,200and400mg/kg urtica reduced the levels of interleukin-1β,tumor necrosis factor-αand matrix metalloproteinase3in the synovial fluid.These results manifest that urtica exerts the effects of anti-inflammation,analgesia,detumescence and anti-osteoarthritis.

关 键 词：组织构建 骨组织工程 荨麻 骨关节炎 白细胞介素1Β 肿瘤坏死因子Α 基质金属蛋白酶3 

分 类 号：R318[医药卫生—生物医学工程]