Bacterial diversity in the sea surface microlayer of the Northern Yellow Sea of China  

中国北黄海微表层海水中细菌多样性（英文）

作　　者：MING Hong-xia FAN Jing-feng WANG Xiao-hui LI Hong-bo ZAN Shuai-jun 明红霞;樊景凤;王小慧;李洪波;昝帅君(国家海洋环境监测中心,辽宁大连116023;国家海洋局近岸海域生态环境重点实验室,辽宁大连116023;大连海洋大学,辽宁大连116023)

机构地区：[1]National Marine Environmental Monitoring Center, Dalian 116023, Liaoning province, China [3]Dalian Ocean University, Dalian 116023, Liaoning province, China

出　　处：《Marine Science Bulletin》2017年第2期1-19,共19页海洋通报（英文版）

基　　金：supported by the Natural Science Foundation of China (No. 41406129);the National Key Research Program (Grant 2016YFA0601400);the State Oceanic Administration of China(Grant GASI-03-01-02-05)

摘　　要：The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this study,we compared the bacterial cell abundance and community structure of the SML and subsurface waters(0.50m depth)using traditional culture and flow cytometry methods.Overall,53strains were screened and sequenced.In addition,DNA was extracted from the SML and corresponding subsurface water and used for bacterial community analysis by denaturing gradient gel electrophoresis(DGGE).The results revealed that the SML had a distinct microbial community structure from the corresponding subsurface water.This is the first compresensively detailed molecular biological analysis of the bacterioneuston in the Yellow Sea of China.微表层是海洋环境与大气对流层之间的一层生物膜,具有独特的海洋生态系统和物理化学、微生物组成。本研究采用传统的培养和流式细胞计数方法,比较了微表层和次表层(0.50米深)的细菌丰度,并对筛选的53株菌进行了测序,分析了可培养细菌的群落结构。此外,直接从微表层和相应次表层海水中提取DNA,通过变性梯度凝胶电泳(DGGE)方法分析了总细菌的群落结构。结果表明,海洋微表层存在一个特殊的生态群,其微生物群落结构明显不同于其相应次水层。本研究采用生态学分子技术全面阐述了中国北黄海微表层海水中的漂浮细菌群落结构。

关 键 词：surface microlayer bacterial diversity denaturing gradient gel electrophoresis 

分 类 号：Q938.8[生物学—微生物学]