高黎贡山丛枝菌根真菌多样性研究  

Diversity of Abuscular Mycorrhizal Fungi(AMF) in Gaoligong Mountain

作　　者：李龙[1,2] 李丽 伍建榕 李博伦[2] 张俊忠 刘丽 马焕成 LI Long;LI Li;WU Jianrong;LI Bolun;ZHANG Junzhong;LIU Li;MA Huancheng(Southwest Key Laboratory of Biodiversity Conservation, State Forestry Administration, Southwest Forestry University, Kunming, Yunnan 650224;Yunnan Key Laboratory of Forest Disaster Warning and Control, Southwest Forestry University, Kunming, Yunnan 650224, China)

机构地区：[1]西南林业大学国家林业局西南地区生物多样性保育重点实验室,云南昆明650224 [2]西南林业大学林学院云南省高校森林灾害预警控制重点实验室,云南昆明650224

出　　处：《贵州农业科学》2017年第12期45-50,共6页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目(31260175);云南省高校基础研究产业化项目(2016CYH14);云南省林学一流学科建设项目(51600625);云南省高等学校森林病虫害综合治理教学团队

摘　　要：为弄清高黎贡山物种多样性的形成原因,通过土壤养分分析、丛枝菌根真菌(AMF)孢子湿筛沉淀法和碱解离-苯胺蓝染色法测定6种植被带宿主树种根系丛枝菌根真菌的侵染程度和侵染率、土壤孢子密度和土壤养分含量,研究丛枝菌根真菌多样性垂直地带性分布规律。结果表明:6种植被带宿主树种中,根系均被不同程度的菌丝侵染,其中,4个植被带的宿主植物形成了明显的菌根(AM),占67%;共分离鉴定出5属14种AMF,分别是球囊霉属(Glomus sp.)、无梗囊霉属(Acaulosporasp.)、硬囊霉属(Sclerocystis sp.)、巨孢囊霉属(Gigasporasp.)及内养囊霉属(Entrophosporasp.),其中,球囊霉属和无梗囊霉属种属于优势属种;不同海拔高度的孢子密度不同,随着海拔的升高,孢子密度呈减少趋势,以暖性针叶林孢子密度最高,与其他植被带宿主植物的孢子密度差异极显著(P<0.01);海拔、土壤有机质含量和矿质元素均可影响丛枝菌根真菌的孢子密度。AMF的丰富度指数受各个植被带树种的多样性、土壤中有机质的含量和土壤中矿质元素含量的影响。The vertical zonality distribution regulation of AMF diversity is determined by means of the AMF infection degree and rate of host trees’roots,soil spore density and soil nutrient content of six vegetation zones analyzed by AMF spore wet screening precipitation,basic dissociation aniline blue staining and soil nutrient analysis to provide a reference for formation causes of species diversity in Gaoligong Mountain.Results:67%host trees’roots of six vegetation zones are infected with hypha to varying degrees.The host plants of four vegetation zones form obvious AM.14species,five genera of AMF are isolated and identified from AM.There are Glomus sp.,Acaulospora sp.,Sclerocystis sp.,Gigaspora sp.and Entrophospora sp.,and Glomus sp.and Acaulospora sp.are dominant genera.The spore density presents a declining trend with altitude increasing.The spore density in warm coniferous forest is the maximum and very significantly higher than other vegetation zones.Altitude,soil organic matter content,and mineral elements all influence AMF spore density.The AMF richness index is influenced by tree species diversity of different vegetation zones,soil organic matter content and soil mineral element content respectively.

关 键 词：丛枝菌根真菌 物种多样性 孢子密度 菌根侵染率 高黎贡山 云南 

分 类 号：S154.3[农业科学—土壤学]