深县猪遗传多样性监测适宜抽样规模的确定  

Determination of Suitable Sampling Size in Genetic Diversity Monitoring of Shenxian Pigs' Population

作　　者：房晓欢 尹彦开 刘洋洋 贾青[1,2,3] 尹雪姣 赵思思[1] 李赛[4] FANG Xiao huan;YIN Yan kai;LIU Yang yang;JIA Qing;YIN Xue jiao;ZHAO Si si;LI Sai(College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000,China;National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China;Engineering Research Center for Agriculture in Hebei Mountainous Areas, Baoding 071000, China;Zheng Nong Animal Husbandry Co., Ltd., Xinji 050000, China)

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]国家北方山区农业工程技术研究中心,保定071000 [3]河北省山区农业工程技术研究中心,保定071000 [4]辛集市正农牧业有限公司,辛集050000

出　　处：《中国畜牧兽医》2017年第11期3250-3256,共7页China Animal Husbandry & Veterinary Medicine

基　　金：河北省现代农业产业技术体系专项(HBCT2013070202);河北省大学生创新创业训练计划项目(201610086028);河北农业大学大学生创新创业训练计划项目(20160401)

摘　　要：为使深县猪保种效果监测更为简化又不失准确性,特建立一种方法以缩减深县猪采样的数量。选取179头深县猪作为一个群体,按照一定的数量梯度抽样,每个梯度随机抽取10次,采用微卫星引物标记的技术计算群体与抽样群的遗传多样性指标。综合10组的遗传多样性评价指标的平均值和标准差与选取的群体对应数值进行比较分析,以遗传多样性变化最小的抽样规模确定为适宜采样规模。结果显示,抽样规模为30头的深县猪遗传多样性主要数据为:平均等位基因数4.6609,平均有效等位基因数3.2325,平均期望杂合度0.6284,平均观察杂合度0.1569,平均PIC0.5794,与整体数据相比,变化较小,具有代表性,可以代替大群测定。A method was created to reduce the sampling number in order to make the preservation monitoring simpler and accurate for the Shenxian pigs’population.A total of179Shenxian pigs as a population was sampled in a certain gradient,each gradient randomly selected10times.The genetic diversity indexes of population and sampling groups were calculated using microsatellite primers marker technique.The average and standard deviation of the genetic diversity indexs of10groups were compared with that of the selected population,and the minimum sampling size with less changes in genetic diversity was determined as the appropriate sampling scale.The results showed that:For the group with sampling size of30,the average allele number was4.6609,the average effective allele number was3.2325,the average observed heterozygosity was0.1569,the average expected heterozygosity was0.6284,the average PIC was0.5794.The results indicated that the main indexes of genetic diversity in group with sampling size of30were similar to those in the whole population,so the sampling size of30pigs could be used to represent and instead of large population of Shenxian pigs.

关 键 词：深县猪 微卫星标记 遗传监测 抽样规模 

分 类 号：Q348[生物学—遗传学]