NAA与6-BA对黑金丝柚木组织培养的影响  

Effects of NAA and 6-BA on Tissue Culture of Tecton grandis

作　　者：李学东 华帅 刘长安[2] LI Xue-dong;HUA Shuai;LIU Chang-an(Menglun Administration of Xishuangbanna State Reserve, Mengla 666303, Yunnan China;Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan China)

机构地区：[1]西双版纳州国家级自然保护区勐仑管护所,云南勐腊666303 [2]中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室,云南勐腊666303

出　　处：《亚热带植物科学》2017年第4期379-382,共4页Subtropical Plant Science

基　　金：国家自然科学基金(31470639)

摘　　要：通过对黑金丝柚木Tecton grandis带潜伏芽茎段的组织培养,研究不同浓度配比6-BA和NAA对组培苗生长的影响。结果表明,黑金丝柚木不定芽分化最佳培养基为MS+0.3 mg·L^(-1) 6-BA+0.6 mg·L^(-1) NAA+30g·L^(-1)蔗糖+12 g·L^(-1)琼脂;生根适宜培养基为MS+0.8 mg·L^(-1) 6-BA+0.5 mg·L^(-1) NAA+30 g·L^(-1)蔗糖+12 g·L^(-1)琼脂;继代培养的最佳培养基为MS+0.5 mg·L^(-1) 6-BA+0.5 mg·L^(-1) NAA+30 g·L^(-1)蔗糖+12 g·L^(-1)琼脂。In this study,effects of different concentration of6-BA and NAA on the growth of in vitro Tecton grandis seedlings were explored.The results showed that the optimum culture medium for adventitious bud differentiation was MS+0.3mg·L-16-BA+0.6mg·L-1NAA+30g·L-1sugar+12g·L-1agar;the best medium for rooting was MS+0.8mg·L-16-BA+0.5mg·L-1NAA+30g·L-1sugar+12g·L-1agar;and the optimum medium for subculture was MS+0.5mg·L-16-BA+0.5mg·L-1NAA+30g·L-1sugar+12g·L-1agar.

关 键 词：黑金丝柚木 组织培养 生长调节剂 分化增殖 

分 类 号：Q943.1[生物学—植物学]