加味千金苇茎汤对大气细颗粒物致肺损伤大鼠肿瘤坏死因子-α和核因子-κB的影响  

Effects of Modified Qianjin Weijing Decoction on TNF-α and NF-κB in Rats with Lung Injury Induced by Particulate Matter

作　　者：沈赵赟 符胜光[1] 杨爱东[1] 吴中华[1] 李素云[1] 帅玉叶 唐普仁 SHEN Zhao-yun;FU Sheng-guang;YANG Ai-dong;WU Zhong-hua;LI Su-yun;SHUAI Yu-ye;TANG Pu-ren(Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国中医药信息杂志》2018年第1期38-42,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学金面上项目(81673855);国家中医药管理局重点学科项目(2009年);上海市重点学科建设资助项目(S30301);上海中医药大学第9批大学生科创资助项目(SHUTCM2016177);上海中医药大学名师研究室项目(A1-Z173020711);上海中医药大学基础医学院与曙光医院院院合作项目(2015年);上海中医药大学德育渗透课题(A1-60102009)

摘　　要：目的观察加味千金苇茎汤对大气细颗粒物致肺组织损伤大鼠肿瘤坏死因子-α(TNF-α)和核因子-κB(NF-κB)的影响,探讨其可能的作用机制。方法大鼠气管滴注15 mg/kg细颗粒物生理盐水混悬液造模,隔日1次,共3次。将32只Wistar大鼠随机分为正常组、模型组和加味千金苇茎汤高、低剂量组,每组8只,给药组每日给予相应剂量药物灌胃。ELISA检测支气管肺泡灌洗液TNF-α水平,免疫组化检测肺组织NF-κB蛋白表达,光镜下观察大鼠肺组织病理变化。结果与正常组比较,模型组大鼠肺泡灌洗液TNF-α含量及肺组织NF-κB蛋白表达显著升高(P<0.01);与模型组比较,加味千金苇茎汤低、高剂量组大鼠肺泡灌洗液TNF-α含量及肺组织NF-κB蛋白表达显著降低(P<0.05,P<0.01)。病理观察结果显示,模型组较正常组小气管内、肺泡及肺间质内有大量细颗粒物沉着,肺泡及肺间质内可见大量吞噬细颗粒物的巨噬细胞并伴有较多中性粒细胞和淋巴细胞浸润;加味千金苇茎汤高、低剂量组肺组织病理改变明显轻于模型组,高剂量组炎症反应较轻,肺泡及肺间质内可见吞噬细颗粒物的巨噬细胞、少量中性粒细胞和淋巴细胞浸润。结论加味千金苇茎汤可能通过降低TNF-α含量、下调NF-κB蛋白表达,以减轻模型大鼠的肺组织损伤,对肺组织有保护作用。Objective To investigate the protective effect of modified Qianjin Weijing Decoction(MQJWJD)on TNF-αand NF-κB in rats with lung injury induced by particulate matter;To discuss relevant mechanism of action.Methods A tracheal drip15mg/kg fine particles of saline solution was used to establish modeling,every other day,three times.Thirty-two Wistar rats were randomly divided into normal group,model group and MQJWJD high-dose and low-dose groups,with eight rats in each group.Medication groups were given relevant medicine for gavage.The level of TNF-αin bronchoalveolar lavage fluid(BALF)was measured by ELISA.The expression of NF-κB protein in lung tissues was measured by immunohistochemistry.The histopathology of the lung injury was observed by light microscope.Results Compared with normal group,the level of TNF-αand the expression of NF-κB protein in the model group were higher than those in the normal group(P<0.01).Compared with model group,the level of TNF-αand the expression of NF-κB protein in MQJWJD low-dose and high-dose groups were lower than those in the model group(P<0.05,P<0.01).Pathological observation showed that,compared with normal group,model group showed intratracheal,alveolar and interstitial bacteria within a large number of fine particles calm,alveolar and pulmonary interstitial visible large amounts of phagocytic fine particles of macrophages and accompanied by more neutrophils and lymphocyte infiltration;Lung tissue pathological changes were significantly lighter in MQJWJD high-dose and low-dose groups than the model group.MQJWJD high-dose group showed mild inflammation,alveolar and pulmonary interstitial visible phagocytic fine particles of macrophages,a small amount of neutrophils and lymphocyte infiltration.Conclusion MQJWJD can reduce the pulmonary injury in rats induced by particulate matter and has protective effects on the rat model through decreasing the levels of TNF-αand the expressions of NF-κB protein in injured lung tissues.

关 键 词：加味千金苇茎汤 细颗粒物 肺损伤 肿瘤坏死因子-Α 核因子-ΚB 大鼠 

分 类 号：R285.5[医药卫生—中药学]