骨骼及牙齿DNA快速提取方法在DVI中的应用  

A Method to Extract Rapidly the DNA from Bones and Teeth for Disaster Victim Identification

作　　者：王传海[1] 李湘秦[1] 吴勇[1] 徐程[1] 杜舟[1] WANG Chuanhai;LI Xiangqin;WU Yong;XU Cheng;DU Zhou(Institute of Criminal Science and Technology of Shenzhen Municipal Public Security Bureau, Shenzhen 518040,Guangdong, China)

机构地区：[1]深圳市公安局刑事科学技术研究所,广东深圳518040

出　　处：《刑事技术》2017年第6期512-514,共3页Forensic Science and Technology

摘　　要：目的探讨骨骼及牙齿DNA快速提取方法在灾难受害者身源鉴定中的应用可行性。方法将滑坡事故中需鉴定身份的43份骨骼、47份牙齿研磨成粉,以King Fisher Duo Prime微量提取系统提取DNA,仅用约3 h即可对单份骨骼或牙齿完成DNA提取。进行STR分型检验。结果测试的90份检材均成功获得STR分型。结论使用King Fisher Duo Prime微量提取系统可以对骨骼及牙齿进行快速DNA提取,适用于灾难受害者的身源鉴定。Objective To explore the feasibility of a rapid method to extract DNA from bones and/or teeth for disaster victim identification(DVI).Methods Ninety bones and teeth of the victims,died from the Shenzhen“2015.12.20”landslide incident,were grounded into powder,from which the DNA was extracted by King Fisher Duo Prime platform within just about3hours and followed to subject to STR genotyping.Results The full-spectrum DNA STR profile was obtained from any one of the90samples.Conclusion For DVI,King Fisher Duo Prime platform is eligible for the rapid extraction of DNA from bones and/or teeth.

关 键 词：法医遗传学 灾难受害者身源鉴定 KingFisherDuoPrime系统 STR分型 骨骼及牙齿 

分 类 号：DF795.2[医药卫生—法医学]