外衣兜内接触性DNA的检验  

STR Test of Touch DNA Extracted from Coat Pockets

作　　者：夏雷 段紫英 陈立彰 富渭鑫 XIA Lei;DUAN Ziying;CHEN Lizhang;FU Weixin(Institute of Forensic Science, Wenzhou Public Security Bureau, Wenzhou 325000, Zhejiang, China)

机构地区：[1]温州市公安局刑事科学技术研究所,浙江温州325200

出　　处：《刑事技术》2017年第6期515-516,共2页Forensic Science and Technology

摘　　要：目的检验3起案件现场衣物3件,探讨此类生物检材的DNA检验策略。方法使用QIAcube核酸自动化提取仪提取脱落细胞DNA,Amp FLSTR~?Identifler~?Plus Kit试剂盒进行STR复合扩增,以ABI3500x L基因分析仪电泳,Gene Mapper~?ID-X软件作基因分型。结果检验的3件外套内兜均成功获得STR分型,相关案件得以侦破。结论根据现场遗留衣物并结合案件情况,采取有效检验策略和手段,可明显提高相关STR分型成功率,为案件侦破提供有力的技术支撑。Objective To explore the strategy about STR test for the DNA evidential materials of exfoliated cells collectedfrom coat pocket that was involved with the crime.Methods DNA,extracted from the exfoliated cells with QIAcubeForensic Casework DNA extraction system,was taken as the template to undergo the PCR amplification and capillaryelectrophoresis for STR genotyping analysis.Results STR genotyping profiles were successfully acquired from each of theseized coat pockets,making the relevant3cases uncovered promptly.Conclusions Biologically evidential materials,such asexfoliated cells from coat or other clothes left at the crime scene,can significantly reveal their STR profiling and powerfullyprovide technical supports for the involved case to be solved when the efficient DNA test strategy was adopted based on thecase situation.

关 键 词：生物物证 接触性DNA DNA检验 外衣 

分 类 号：DF795.2[医药卫生—法医学]