赤芍药材中芍药苷含量测定方法的改进  

Improvement of the Determination Method of Paeoniflorin in Radix Paeoniae Rubra

作　　者：张艳[1] 申丽莎[1] 杨帆 王祯旭 邓开英 Zhang Yan;Shen Lisha;Yang Fan;Wang Zhenxu;Deng Kaiying(Chongqing Academy of Chinese Materia Medica, Chongqing, China 400061;Chongqing Institute for Food and Drug Control, Chongqing,China 401121;Chongqing Engineering Center for Pharmaceutical Process and Quality Control, Chongqing, China 401121)

机构地区：[1]重庆市中药研究院,重庆400061 [2]重庆市食品药品检验检测研究院,重庆401121 [3]重庆市药物过程与质量控制工程技术研究中心,重庆401121

出　　处：《中国药业》2017年第24期28-30,共3页China Pharmaceuticals

摘　　要：目的对测定赤芍药材中芍药苷含量的高效液相色谱(HPLC)法进行优化。方法色谱柱采用Waters Sun Fire C18柱(150 mm×4.6 mm,5μm),以乙腈-0.1%磷酸(14∶86)为流动相,流速为2.0 m L/min,检测波长为230 nm。结果芍药苷进样量在0.061 04~1.220 8μg范围内与峰面积积分值线性关系良好(r=0.999 9),平均加样回收率为97.65%,RSD为1.47%(n=6)。结论该方法简便,快速,结果准确,可用于赤芍药材的质量控制。Objective To improve the HPLC method for the content determination of paeoniflorin in Radix Paeoniae rubra.Methods The Waters SunFire Ci8column(150mm x4.6mm,5(xm)was adopted with the mobile phase of acetonitrile-0.1%phosphoric acid(14:86),the flow rate was2.0m L/m in,and the detection wavelength was230nm.Results The linear range of paeoniflorin was0.06104-1.2208fxg(r=0.9999),the average recovery was97.65%,the RSD was1.47%(m=6).Conclusion The method is simple,rapid and accurate,which can be used for the quality control of Radix Paeoniae rubra

关 键 词：赤芍药材 芍药苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R282.6[医药卫生—中医学]