香烟烟雾暴露对雄性大鼠血浆尼古丁、可替宁的影响  

Effect of cigarette smoking-induced plasma nicotine and cotinine on male rats

作　　者：何丽娟[1] 仲春雪 张静[1] 张晨[1] 黄云飞[1] 邹莹[1] HE Li-juan;ZHONG Chun-xue2;ZHANG Jing;ZHANG Chen;HUANG Yun-fei;ZOU Ying(College of Public Health,Xinjiang Medical University,Urumqi830054, China;The Fifth Affiliated Hospital,Xinjiang Medical University, Urumqi830054)

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830054 [2]新疆医科大学附属第五医院,乌鲁木齐830054

出　　处：《中国实验动物学报》2017年第6期594-599,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(No.81260433);新疆研究生科研创新项目(No.XJGRI2015067);乌鲁木齐市优秀青年科技人才项目(No.2016SKJRC-2)

摘　　要：目的通过对吸烟大鼠血浆尼古丁、可替宁含量的测定,探讨香烟在体内的内暴露水平。方法清洁级SD雄性大鼠160只,随机分为2、4、6、8、12周的低、中、高染毒剂量组及对照组,每组10只。采用呼吸道静式染毒,每日染毒一次,染毒周期为2、4、6、8、12周。观察大鼠的一般毒性效应,运用气相色谱-质谱联用法检测吸烟大鼠血浆中尼古丁、可替宁的含量。结果香烟烟雾暴露组大鼠在第3周后逐渐开始出现间歇咳嗽,腹式呼吸加剧,鼻噪音等症状,暴露时间越长,症状越明显;染毒剂量越高,染毒周期越长,大鼠体重减少越明显,与空白对照组相比,差异有显著性(P<0.01);大鼠血浆尼古丁的保留时间为7.5~8.5 min,随暴露时间的延长,暴露剂量的增加,大鼠血浆中尼古丁含量较空白对照组(155±56.65)ng/m L有所增加;大鼠血浆可替宁的保留时间为11.5~12.5 min;随暴露时间的延长,暴露剂量的增加,大鼠血浆中可替宁含量较空白对照组(340±41.97)ng/m L有所增加,且呈现剂量-反应关系,不同暴露周期间可替宁含量的差异有显著性(P<0.05),且暴露时间与暴露剂量存在交互效应(P<0.05)。结论香烟烟雾暴露可造成大鼠机体不同程度的损伤,大鼠血浆可替宁浓度能够有效反映香烟烟雾内暴露情况,且呈现较好的剂量-反应关系。Objective To explore the in vivo exposure levels of cigarette smoking(CS)by measuring the biomarkers nicotine and cotinine.Methods One hundred and sixty male SD rats were divided into15cigarette exposure groups(10,20and30nonfilter cigarettes/day,for2,4,6,8and12weeks)and a control group(without CS exposure).The rats were sacrificed at different time-points.The concentration of plasma nicotine and cotinine were measured by GC-MS/MS.Results The CS-exposed rats displayed decreased locomotor activity,ataxic gait,irregular respiration,nasal noise,and salivation after smoking exposure for3weeks.Rats in the CS exposure groups had lower body weight,and the reduction of body weight was time and dose related(P<0.01).The retention time of nicotine was7.5to8.5min.The concentration of plasma nicotine in the CS exposure groups was higher than control group(155±56.65)ng/mL.The retention time of cotinine was11.5to12.5min,the concentrations of plasma cotinine in CS exposure groups were higher than control group(340±41.97)ng/mL,the increase of plasma cotinine in CS groups was time?related(P<0.05),and the exposure concentration and duration had synergistic effect on the level of plasma cotinine(P<0.05).Conclusions CS exposure causes structural damages in male rats.The plasma concentration of cotinine can effectively reflect the in vivo exposure levels of cigarette smoking,and well presents a dose?response relationship.

关 键 词：香烟烟雾暴露 动物模型 尼古丁 可替宁 

分 类 号：Q95-33[生物学—动物学]