不同感染途径致大鼠肺炎模型制备的比较  

Comparison on the preparation of rat models of pneumonia by different infection route

作　　者：丁军颖 高翔[2] 洪燕英[2] 桂红[2] 韦志友 丁雪霏 卢幼然 安世栋 崔煦然 郭玉红[2] 刘清泉 DING Jun.ying;GAO Xiang;HONG Yan.ying;GUI Hong;WEI Zhi-you;DING Xue-fei;LU You-ran;CUI Xu-ran;GUO Yu-hong;LIU Qing-quan(Beijing Key Lab of Basic Study on TCM Infectious Diseases, Beijing Hospital of TCM, Capital Medical University, Beijing Institute of TCM, Beijing100010, China;Beijing Hospital of TCM, Capital Medical University, Beijing100010)

机构地区：[1]首都医科大学附属北京中医医院北京市中医研究所中医感染性疾病基础研究北京市重点实验室,北京100010 [2]首都医科大学附属北京中医医院,北京100010

出　　处：《中国实验动物学报》2017年第6期600-604,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(No.81503399;No.81373813);国家"重大新药创制"科技重大专项(No.2013ZX09102026);首都医科大学附属北京中医医院院级课题(No.YJ-201734);首都医科大学基础-临床科研合作基金(No.16JL74)

摘　　要：目的探索铜绿假单胞菌致大鼠肺炎模型的制备方法,为后续研究奠定实验基础。方法将Sprague-Dawley(SD)大鼠48只随机分为4组:A对照组、B气管注射组、C气管插管感染组和D滴鼻感染组。适应饲喂3 d后,分别给予不同造模干预,分别于干预后5、10和15 d动态监测各亚组大鼠体重、体温、白细胞数、肺组织病理学变化等。结果各模型组大鼠行为学表现、体重、体温、白细胞、肺组织炎症病理学表现均与对照组差异有显著性;(2)各模型组均证实为铜绿假单胞菌感染,对照组则阴性。结论经滴鼻途径以铜绿假单胞菌感染大鼠,可得到合格大鼠肺炎模型;此感染途径可避免手术创口带来的炎症反应干扰,简便易行,可推广。Objective To explore the preparation of a rat model of pneumonia model induced by Pseudomonas aeruginosa(PA)using different methods,and to lay the foundation for further studies.Methods48SD rats were randomly divided into4groups:the control group(A),the intratracheal injection group(B),the trachea cannulation group(C)and the intranasal inoculation group(D).After intervention with different treatment modalities,the body weight,temperature,white blood cell count and lung pathological changes in the rats of all groups were detected at5,10,15days.Results1.The behavior,body weight,temperature,leukocytes and pathological inflammatory changes of the lung in rats of the model groups were significantly different from that of control group.2.Pseudomonas aeruginosa was detected in rats of all the model groups,but the control group was negative.Conclusions Rat model of Pseudomonas aeruginosa infected pneumonia can be successfully established by intranasal inoculation.This method can avoid the inflammatory interference from operation,and is simple and suitable for popularization.

关 键 词：铜绿假单胞菌 耐药菌 肺炎 炎症反应 大鼠 

分 类 号：Q95.33[生物学—动物学]