长爪沙鼠体外受精与早期胚胎体外培养体系的初步建立  

Establishment of a modified in vitro mongolian gerbil oocyte fertilization and embryo culture system

作　　者：郭红刚[1] 李莉[2] 周生来 卢领群[1] 李坤[1] 杜江涛[1] 石巧娟[1] 金秀清 李长龙[4] 萨晓婴[1] 应华忠[1] 褚晓峰[1] GUO Hong-gang;LI Li;ZHOU Sheng-lai;LU Ling-qun;LI Kun;DU Jiang-tao;SHI Qiao-juan;JIN Xiu-qing;LI Chang-long;SA Xiao-ying;YING Hua-zhong;CHU Xiao-feng(Zhejiang Academy of Medical Sciences, Zhejiang Experimental Animal and Safety Research Laboratory, Hangzhou310013, China;Hangzhou Disease Prevention and Control Center, Hangzhou3100212;Experimental Animal Department of China Medical University, Shenyang110001;Capital Medical University Basic Medical College,Beijing100069)

机构地区：[1]浙江省医学科学院,浙江省实验动物与安全性研究重点实验室,杭州310013 [2]杭州市疾病预防控制中心,杭州310021 [3]中国医科大学实验动物部,沈阳110001 [4]首都医科大学基础医学院,北京100069

出　　处：《中国实验动物学报》2017年第6期624-631,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家科技支撑计划子课题(No.2015BA109B01-02);浙江省级公益性技术应用研究计划(No.2015C37119);浙江省卫生厅课题(No.2015KYB093)

摘　　要：目的探讨自配的获能液和受精液用于长爪沙鼠体外受精的可行性,为长爪沙鼠的胚胎保种提供参考。方法用自配的获能液和受精液对长爪沙鼠进行体外受精,并用改良后的KSOM培养液对长爪沙鼠的2细胞胚胎进行体外培养试验。结果长爪沙鼠体外受精率在60%以上,沙鼠2细胞胚胎能够在体外进一步发育。结论初步建立了长爪沙鼠体外受精和胚胎发育体系,但需要进一步优化。ObjectiveTo investigate the feasibility of self made capacitation liquid for in vitro fertilization of Mongolian gerbils,and to provide a reference for gerbil embryo cryopreservation.MethodsIn vitro fertilization of Mongolian gerbil was performed with the self prepared capacitation solution and semen,and the2-cell embryos of Mongolian gerbils were cultured in vitro using an improved KSOM culture medium.ResultsThe in vitro fertilization rate of gerbils was over60%,and some gerbil2-cell embryos could develop further in vitro.ConclusionsAn in vitro fertilization and embryo development system of Mongolian gerbil has been established,but it needs further optimization.

关 键 词：长爪沙鼠 体外受精 体外培养 

分 类 号：Q95-33[生物学—动物学]