无菌猪的制备与微生物质量控制  

Breeding and microbiological quality control of germ-free pigs

作　　者：孙静[1,2,3] 杜蕾 丁玉春[1,2,3] 吴梦[1,2,3] 罗林[1,2,3] 杨松全 黄勇[1,2,3] 曹浩然 钟浩[5] 刘作华 葛良鹏[1,2,3] SUN Jing;DU Lei;DING Yu-chun;WU Meng;LUO Lin;YANG Song-quan;HUANG Yong;CAO Hao-ran;ZHONG Hao;LIU Zuo-hua;GE Liang-peng(Chongqing Academy of Animal Husbandry Sciences, Chongqing402460, China;Key Laboratory of Pig Industry Science, Ministry of Agriculture, Chongqing402460;Chongqing Key Laboratory of Pig Industry Sciences,Chongqing402460;Chongqing BEST Bio.Technology Company Limited, Chongqing402460)

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]农业部养猪科学重点实验室,重庆402460 [3]重庆市养猪科学重点实验室,重庆402460 [4]西南大学动物科学学院,重庆402460 [5]重庆百斯达生物科技有限公司,重庆402460

出　　处：《中国实验动物学报》2017年第6期699-702,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家重点研发计划项目(No.2017YFD0501600);重庆市社会事业与民生保障科技创新专项项目(No.17306);重庆市基本科研业务费项目(No.16422);重庆市农发资金项目(No.17408)

摘　　要：菌群与宿主相互关系等热点研究促进了对无菌动物需求的迅猛发展。无菌猪作为一种特殊的模式动物,其在科学研究中不仅排除了背景微生物因素的影响,而且与常用的无菌小鼠相比,其在解剖、生理、代谢等方面与人更为相似,因此在生命科学研究领域具有重要的应用价值。本文结合现有国外无菌猪研究进展和本实验室在无菌猪研究积累,就无菌猪的制备与微生物质量控制进行简要探讨。The industry of germ free animals has been a hot spot in research along with the rapid development of studies on the relationship between microbiota and host diseases.Because it is pathogen free,and the high degree of similarity in anatomy,physiology,pathogenesis to humans,germ free pig is considered a clinical relevant model to be widely used in life science research.Based on the current state of research of germ free pig cultivation at home and abroad and the experimental studies carried out in our laboratory as well,this article gives a simple discussion on germ free technique of domestic pigs.

关 键 词：无菌猪 屏障环境 饲养隔离器 微生物学监测 

分 类 号：Q95-33[生物学—动物学]