基于线粒体多基因串联序列的蚬蝶类系统发生关系分析  

Phylogenetic Placement of Riodinids(Lepidoptera:Papilionidae) Based on Multiple Mitochondrial Gene Sequences

作　　者：石庆会 张桂清 韦中学 吴楠 张严[1,2,3] SHI Qing-hui;ZHANG Gui-qing;WEI Zhong-xue;WU Nan;ZHANG Yan(School of Resources and Chemical Engineering, Sanming University, Sanming 365004,China;Fujian Provincial Key Laboratory of Resources and Environment Monitoring and Sustainable Management and Utilization, Sanming University, Sanming 365004, China;Fujian Province Engineering Research Center of Mine Ecological Construction,Sanming University, Sanming 365004,China)

机构地区：[1]三明学院资源与化工学院,福建三明365004 [2]福建省资源环境监测与可持续经营利用重点实验室,福建三明365004 [3]福建省矿山生态修复工程技术研究中心,福建三明365004

出　　处：《三明学院学报》2017年第6期9-17,共9页Journal of Sanming University

基　　金：福建省教育厅中青年教师教育科研项目(JAT160456);三明学院科学研究基金(B201605);福建省科技厅自然科学基金项目(2017J05059);福建省自然科学基金项目(2014J01136)

摘　　要：测定了蚬蝶类两个代表种(波蚬蝶、银纹尾蚬蝶)的线粒体基因16S r RNA、Cytb、COI、COIII的部分序列。同时,结合Gen Bank数据库中已公布的其他蝶类52个代表种相应的基因序列,选取蛾类2个代表种作为外类群。基于这4个基因的串联序列,分别使用邻接法、最大简约法、最大似然法和贝叶斯推论法重新构建了蝶类的分子系统树,分析了蚬蝶类的系统发生关系。序列分析结果显示:经比对处理后串联序列共有4784个同源位点,其中保守位点2113个,变异位点2669个,简约信息位点2149个;A+T平均含量为76.8%,具有明显的AT偏倚性。分子系统树表明:蚬蝶类形成一个单系群,并与灰蝶科形成姊妹群,结果进一步表明蚬蝶类与灰蝶科具有较近的亲缘关系,但能否归入灰蝶科还有待于进一步的论证。T he partial mitochondrial16S rR N A,C ytb,C O I and C O I II genes of Zemeros flegyas and Dodona eugenes were newly amplified and sequenced.M eanw hile,the homologous sequences of52representative species of butterfly were obtained from the G enB ank.B ased on these data,the sequence variation and the phylogenetic relationship of these groups were analyzed,and the phylogenetic trees of the54species from all currently recognized families of butterfly were reconstructed based the combined dataset of the four gene sequences with the neighbor joining(N J),maximum parsimony(M P),maximum likelihood(M L)and B ayesian Inference(B I)methods.T he results of the sequence analysis showed that the four combined genes are4784bp in length by alignment,including2113conserved sites,2669variable sites and2149parsimonious-informative sites,and the average percentage of A+T is76.8%.T he results of phylogenetic analyses indicated that the riodinid butterflies w ere strongly supported monophyletic groups and sister to L ycaenidae.T herefore,the results of this paper further confirmed that the R iodinidae and L ycaenidae are more closely to each other.H ow ever,it is needed to further prove whether it belongs to L ycaenidae.

关 键 词：鳞翅目 蚬蝶科 线粒体基因 系统发生关系 

分 类 号：Q969.432.1[生物学—昆虫学]