负载抗菌药物骨水泥的药物释放实验研究  

The drug release characteristics of antibiotic-loaded polymethyl methacrylate cements

作　　者：吕静[1] 叶盛英[2] 王昊[3] 郝建华 满淑丽[4] 谢宝钢[3] Lv Jing;Ye Shengying;Wang Hao;Hao Jianhua;Man Shuli;Xie Baogang(Department of Nephrology,the 254 Hospital of PLA,Tianjin300142;Department of Phannacy,the 254 Hospital of PLA,Tianjin300142;Department of Orthopaedics,the 254 Hospital of PLA,Tianjin300142;College of Biotechnology,Tianjin University of Science & Technology,Tianjin300457, China)

机构地区：[1]天津解放军第254医院肾内科,天津300142 [2]天津解放军第254医院药剂科,天津300142 [3]天津解放军第254医院骨科,天津300142 [4]天津科技大学生物工程学院,天津300457

出　　处：《生物骨科材料与临床研究》2017年第6期7-10,共4页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的揭示负载抗菌药物骨水泥的药物释放浓度、持续时间、药物残留程度及低于最小有效抑菌浓度的释放时间,为合理应用抗菌药物提供理论依据。方法通过对负载盐酸万古霉素和亚胺培南西司他丁钠的骨水泥进行体外洗提实验,采用高效液相色谱法检测分析两种负载抗菌药物的骨水泥的药物释放浓度、释放持续时间和药物残留程度。结果 (1)负载抗菌药物的骨水泥第一天药物释放量最大,后逐渐持平,且释放时间较长,释放浓度较低;(2)截止到第7周盐酸万古霉素、亚胺培南西司他丁释放量分别只占总负载量的15%、25%;(3)盐酸万古霉素第1周后释放浓度低于最小有效抑菌浓度,亚胺培南西司他丁钠第7周后释放浓度低于最小有效抑菌浓度。结论载抗菌药物骨水泥存在长时间药物残留现象,是导致持续低于最小有效抑菌浓度的主要原因。此种现象不但对细菌起不到抑菌作用,而且是诱导细菌耐药性的危险因素。Objective The effects of the drug concentration,release period,the residue concentration,and releasing time that under the minimal inhibitory concentration from polymethyl methacrylate(PMMA)cement will be studied,in order to lay down a theoretical basis for rational use of antibacterial drugs.Methods Imipenem or vancomycin loaded in the PMMA cement samples was eluted in vitro.High performance liquid chromatography(HPLC)was used to quantitatively determine the releasing profile and residue of imipenem or vancomycin in the antibiotic-loaded PMMA samples.Results①Antibiotics rapidly released from the PMMA samples on the first day,and slowly released at a low concentration afterwards;②Only15%of vancomycin and25%of imipenem were separately released from the antibiotic-loaded PMMA samples after elution for seven weeks.Therefore,there were a large amount of antibiotics remained in the PMMA;③The drug releasing concentration from vancomycin-loaded PMMA was lower than the minimal inhibitory concentration after one week,while that from imipenem-loaded PMMA was lower than the minimal inhibitory concentration after seven weeks.Conclusion The drug releasing concentration from antibiotic-loaded PMMA samples was lower than the minimum bacteriostatic concentration,due to a large amount of residues in the PMMA,which likely results in antibiotics resistance for bacteria

关 键 词：骨水泥 药物控释 抗菌药物残留 

分 类 号：R318.08[医药卫生—生物医学工程]