藏红T共振光散射法测定痕量双酚A方法  

Study on determination of trace amount of bisphenol A by resonance light scattering with safranine T

作　　者：汤健彬 吕昌银[2] 唐宏兵[1] 欧阳运富[1] 石飞云[1] TANG Jian bin;LV Chang yin;TANG Hong bing;OUYANG Yun fu;SHI Fei yun(Changzhou Municipal Center for Disease Control and Prevention, Changzhou 213022,China)

机构地区：[1]江苏省常州市疾病预防控制中心,常州213022 [2]南华大学公共卫生学院

出　　处：《江苏预防医学》2017年第6期612-613,618,共3页Jiangsu Journal of Preventive Medicine

基　　金：常州市科技支撑计划(社会发展)(CE20165042)

摘　　要：目的建立一种共振光散射法测定痕量双酚A方法。方法在pH8.0的B-R缓冲溶液中,KIO4-藏红T-双酚A体系发生氧化还原及离子缔合反应,使藏红T在波长332nm和560nm处的共振光散射明显增强,据此建立一种共振光散射法测定样品中痕量双酚A的含量。结果共振光散射增强值(ΔI)与双酚A浓度(ρ)之间呈良好的线性关系。在332nm处,方法的线性范围为1.43×10^(-4)~2.40μg/mL,检出限为0.043μg/L;在560nm处,方法的线性范围为5.70×10^(-4)~3.60μg/mL,检出限为0.17μg/L。结论建立的方法简便快速、灵敏度高,用于塑料薄膜和矿泉水瓶浸提液中双酚A的测定,结果满意。ObjectiveTo develop a novel resonance light scattering(RLS)method for determination of trace amount of bisphenol A(BPA)MethodsIn pH80Britton Robinson buffer solution,the RLS value of safranine T was significantly enhanced at332nm and560nm due to the redox and ion association reaction in KIO4safranine T BPA system A resonance light scattering method was established for the determination of trace amount of BPA ResultsThere was a good linear relationship between resonance scattering enhancement value(ΔI)and BPA concentration(ρ)At332nm,the linear relationship range of the method was143×10-4-240μg/mL with the detection limit of0043μg/L At560nm,the linear relationship range of the method was570×10-4-360μg/mL with the detection limit of017μg/L ConclusionThe established method was simple,rapid and sensitive Application on food packing and mineral water bottle extracts to determine BPA achieved satisfactory results.

关 键 词：双酚A 藏红T 共振光散射 高碘酸钾 

分 类 号：R195[医药卫生—卫生统计学]