补肺活血胶囊中毛蕊异黄酮葡萄糖苷、毛蕊异黄酮的测定方法  

Determination of Calycosin-7-O-β-D-glucoside and Calycosin in Bufeihuoxue Capsules by HPLC

作　　者：唐清 朱晓枭 郑玉莹 关敏怡 彭维[1] 苏薇薇[1] TANG Qing;ZHU Xiaoxiao;ZHENG Yuying;GUAN Minyi;PENG Wei;SU Weiwei(School of Life Sciences, Sun Yat sen University, Guangzhou 510275, China)

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2017年第6期123-127,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省中医药局科研课题(20161050)

摘　　要：采用HPLC构建测定补肺活血胶囊中毛蕊异黄酮葡萄糖苷和毛蕊异黄酮的方法。以Hitachi High-Tech C_(18)(5μm,4.6 mm×250 mm)为色谱柱,以乙腈-φ=0.2%甲酸溶液为流动相,梯度洗脱,检测波长为260 nm,色谱柱温度为25℃,流速为0.8 m L/min。经方法学验证,毛蕊异黄酮葡萄糖苷、毛蕊异黄酮分别在0.022 5~0.901 8μg(R2=1)、0.007 5~0.299 0μg(R^2=1)范围内线性关系良好,二者的平均回收率分别为93.97%(RSD=3.46%)、90.04%(RSD=3.25%)。结果表明,所建立的方法简单、快捷,适用于补肺活血胶囊中毛蕊异黄酮葡萄糖苷和毛蕊异黄酮的测定。The HPLC method was established for determination of Calycosin7OβD glucoside and Calycosin in Bufeihuoxue Capsules.The separation was performed on Hitachi High Tech C18(5μm,46mm×250mm)column eluted with the mobile phase consisting of acetonitrile and02%formic acid solution in gradient elution mode at the flow rate of08mL/min.The detection wavelength was260nm and the column temperature was25℃.The method was validated and shown that Calycosin7OβD glucoside and Calycosin had a good linear relationship in the range of00225~09018μg(R2=1),00075~02990μg(R2=1),respectively.The average recovery rates were9387%(RSD=346%)、9004%(RSD=325%),respectively.The established method is simple,rapid and reproducible,which is suitable for the determination of Calycosin7OβD glucoside and Calycosin from Bufeihuoxue Capsules.

关 键 词：高效液相色谱 补肺活血胶囊 毛蕊异黄酮葡萄糖苷 毛蕊异黄酮 测定 

分 类 号：R917[医药卫生—药物分析学]