艾滋病合并真菌感染患者NK细胞的亚群分布及细胞因子表达分析  

Study on the distribution of NK cell subsets and cytokine expression in ADIS patients with fungal infection

作　　者：柳丽娟[1] 赵敏 郑丽萍[1] 卓传尚[1] 黃爱琼 LIU Lijuan;ZHAO Min;ZHENG Liping;ZHUO Chuanshang;HUANG Aiqiong(Infectious Diseases Hospital of Fuzhou City, Fuzhou 350025, Fujian, China)

机构地区：[1]福建省福州市传染病医院,福建福州350025

出　　处：《现代中西医结合杂志》2017年第36期4010-4013,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：福建省福州市科技计划项目(2013-S-125-2)

摘　　要：目的分析艾滋病合并真菌感染患者NK细胞的亚群分布及细胞因子表达情况。方法收集并分离61例艾滋病合并深部真菌感染者(病毒RNA为阳性,HIV+IFI组)、30例抗逆转录病毒治疗后艾滋病者(病毒RNA为阴性,HIV+Non-IFI组)、26例健康体检者(健康对照组)的外周血单个核细胞和血清/血浆,并应用流式细胞术(FCM)检测NK细胞亚群分布,应用悬液芯片技术检测白细胞介素-6(IL-6)、IL-8、IL-10、肿瘤坏死因子-α(TNF-α)水平,分析CD4^+、CD8^+、CD4^+/CD8^+、NK细胞亚群分布与HIV-1 RNA、细胞因子表达的相关性。结果与HIV+Non-IFI组、健康对照组比较,HIV+IFI组患者CD56dimNK占NK细胞总数的比例、NK细胞总数及NK亚群细胞数量均显著降低(P均<0.05),而CD56-CD16+NK占NK细胞总数的比例明显升高(P<0.05)。HIV-1 RNA载量与CD4^+T细胞、CD4^+/CD8^+、CD56dimNK、NK呈负相关(P<0.05),与CD8^+T细胞、CD56brightNK、CD56-CD16+NK无相关性(P>0.05)。TNF-α与CD56bright NK、CD56dimNK呈负相关(P<0.05);IL-10、IL-6与CD4^+T细胞、CD56brightNK、CD56dimNK呈负相关(P<0.05);IL-8与CD56-CD16+NK正相关(P<0.05)。结论艾滋病合并真菌感染改变了NK细胞亚群的构成,且该NK细胞亚群的构成变化可调节HIV/真菌共感染患者体内细胞因子应答。Objective It is to analyze the distribution of NK cell subsets and cytokine expression in ADIS patients with fungal infection.Methods After the peripheral blood was collected from61ADIS patients with fungal infection(Viral RNA was positive,HIV+IFI group),30ADIS patients with antiretroviral therapy(Viral RNA was negative,HIV+Non IFI group),and26cases of healthy controls(control group),mononuclear cells and serum/plasma were separated from these blood samples.The distribution of NK cell subsets was detected by Flow cytometry,and the expression of IL6,IL8,IL10,TNFαwas measured by suspension chip technology.The relationship between CD4+,CD8+,CD4+/CD8+,the distribution of NK cell subsets and the expression of HIV1,RNA and cytokines was analyzed.Results Compared with HIV+Non-IFI group and control group,the total number of NK cells and the number of subgroup of NK cells were significantly decreased(all P<0.05),and the CD56dim NK proportion of the total number of NK cells was dramatically down regulated(P<0.05),but the CD56-CD16+NK proportion of the total number of NK cells was obviously increased(P<0.05).HIV-1RNA load was negatively correlated with CD4+T cell,CD4+/CD8+,CD56dim NK proportion and NK cells number(all P<0.05),and had no correlation with CD8+T cell,CD56bright NK,CD56-CD16+NK proportion(all P>0.05).The level of TNFαwas negatively correlated with CD56bright NK and CD56dim NK(P<0.05).The levels of IL10and IL6were negatively correlated with CD4+T cells,CD56bright NK and CD56dim NK(all P<0.05),meanwhile IL8was positively correlated with CD56-CD16+NK(P<0.05).Conclusion The distribution of NK cell subsets is changed by HIV merged fungal infection,which further regulate the cytokine response in ADIS patients with fungal infection.

关 键 词：艾滋病 真菌感染 NK细胞 细胞因子 

分 类 号：R512.91[医药卫生—内科学]