毛脉酸模正丁醇部位对内毒素所致小鼠急性肺损伤的影响  

Effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury in mice

作　　者：欧燕[1] 商思阳[2] 戴艺[1] Ou Yan;Shang Si-yang;Dai Yi(Chongqing Qijiang People's Hospital, Chongqing, 401420;Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021)

机构地区：[1]重庆市綦江区人民医院,重庆401420 [2]重庆市中医院,重庆400021

出　　处：《海南医学院学报》2017年第24期3331-3334,共4页Journal of Hainan Medical University

基　　金：重庆市科技厅民生科研基金(120990)~~

摘　　要：目的:研究毛脉酸模(Rumex gmelini Turcz)正丁醇部位对内毒素所致小鼠急性肺损伤(ALI)的影响。方法:选择昆明种小鼠作为实验动物,并随机分为空白对照组(NC组)、急性肺损伤组(ALI组)、毛脉酸模正丁醇组(Rum组)。ALI组和Rum组采用内毒素腹腔注射的方式建立ALI模型,Rum组在造模前给予毛脉酸模正丁醇部位灌胃干预。注射内毒素12h后,取右肺上叶并测定含水量,取右肺下叶并测定AQPs分子、炎症反应分子、氧化应激分子的含量。结果:注射内毒素12h后,Rum组小鼠肺组织的含水量为(6.82±0.97)%。经方差分析,ALI组小鼠肺组织的含水量显著高于NC组(P<0.05),肺组织AQP1、AQP5的蛋白含量显著低于NC组(P<0.05),AQP3、AQP4的蛋白含量与NC组比较无差异(P>0.05),MPO、NF-kB、TNF-α、HMGB1、IL-8、ROS、ATP、MDA、Bax、Caspase-3的蛋白含量显著高于NC组(P<0.05);Rum组小鼠肺组织的含水量显著低于ALI组(P<0.05),肺组织AQP1、AQP5的蛋白含量显著高于ALI组(P<0.05),AQP3、AQP4的蛋白含量与ALI组比较无差异(P>0.05),MPO、NF-kB、TNF-α、HMGB1、IL-8、ROS、ATP、MDA、Bax、Caspase-3的蛋白含量显著低于ALI组(P<0.05)。结论:毛脉酸模正丁醇部位能够减轻内毒素所致小鼠ALI过程中的肺水肿、炎症反应、氧化应激及细胞凋亡。Objective:To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury(ALI)in mice.Methods:Kunming mice were selected as experimental animals and randomly divided into normal control group(NC group),acute lung injury group(ALI group)and n-butanol extract of Rumex gmelini Turcz group(Rum group).ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin,and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment.12h after endotoxin injection,the superior lobe of right lung was taken to determine the water content,and the inferior lobe of right lung was taken to determine the contents of AQPs molecules,inflammatory response molecules and oxidative stress molecules.Results:12h after endotoxin injection,the water content of lung tissue in Rum group was(6.82±0.97)%.After variance analysis,the water content of lung tissue in ALI group was significantly higher than that in NC group,AQP1and AQP5protein levels in lung tissue were significantly lower than those of NC group,AQP3and AQP4protein levels were not different from those of NC group,and MPO,NF-kB,TNF-α,HMGB1,IL-8,ROS,ATP,MDA,Bax and Caspase-3protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group,AQP1and AQP5protein levels in lung tissue were significantly higher than those of ALI group,AQP3and AQP4protein levels were not different from those of ALI group,and MPO,NF-kB,TNF-α,HMGB1,IL-8,ROS,ATP,MDA,Bax and Caspase-3protein levels were significantly lower than those of ALI group.Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema,inflammatory response,oxidative stress and apoptosis during the endotoxin-induced ALI in mice.

关 键 词：毛脉酸模 内毒素 急性肺损伤 炎症反应 氧化应激 

分 类 号：R563[医药卫生—呼吸系统]