转LpP5CS和LpP5CSF128A基因烟草耐盐及抗旱性分析  

Analysis of resistance to salt and drought of transgenic LpP5CS and LpP5CSF128A's tobacco

作　　者：曹丽[1] 韩蕾[2] 王彩凤 孙振元[2] CAO Li;HAN Lei;WANG Caifeng;SUN Zhenyuan(Yanbian University,Yanji Jilin 133002,China;State Key Laboratory of Tree Genetics and Breeding, ResearchInstitute of Forestry, Chinese Academy of Forestry,Beijing 100091, China;Examing and Inspection Center for Agricultural Products Safety and Quality of Taobei District in Baicheng City, Baicheng Jilin 137000, China)

机构地区：[1]延边大学,吉林延吉133002 [2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [3]白城市洮北区农产品质量安全监督检测中心,吉林白城137000

出　　处：《延边大学农学学报》2017年第4期17-22,43,共7页Agricultural Science Journal of Yanbian University

基　　金：国家"863"项目(2011AA100209);吉林省教育厅项目(吉教科合字[2013]第23号)

摘　　要：利用农杆菌介导法,将多年生黑麦草Δ′-吡咯琳-5-羧酸合成酶基因LpP5CS和该酶第128位苯丙氨酸变异为丙氨酸的突变基因LpP5CSF128A转入烟草,获得转基因表达植株。在NaCl和自然干旱处理下,对多年生黑麦草的野生型基因LpP5CS和突变基因LpP5CSF128A的抗逆生理功能进行初步研究,通过测定脯氨酸(Pro)、丙二醛(MDA)含量和T1代烟草幼苗耐盐性鉴定,进一步证实LpP5CS和LpP5CSF128A过表达的转基因烟草植株,其耐盐性和抗旱性得到了明显改善,转LpP5CSF128A植株的胁迫耐性要强于转LpP5CS植株。Transgenic plants of tobacco were obtained by the agrobacterium mediated transformation of perennial ryegrass LpP5CS and LpP5CSF128A genes.The physiological functions of stress resistance for LpP5CS and LpP5CSF128A genes were preliminarily studied under NaCl and natural drought stress.The results showed that the determination of proline(Pro)and malondialdehyde(MDA)contents and salt tolerance of seedlings revealed that salt and drought tolerances of T1generation tobacco seedlings transformed LpP5CS and LpP5CSF128A genes were obviously improved;and,tolerances to salt and drought stress of LpP5CSF128A transgenic plants were significantly higher than LpP5CS plant.

关 键 词：转基因烟草 LpP5CS基因 LpP5CSF128A基因 耐盐性 抗旱性 

分 类 号：S572[农业科学—烟草工业]