原发性干燥综合征气阴两虚证外周血单个核细胞中miR-155表达水平的研究  

The expression level of miR-155 in peripheral blood mononuclear cell under primary sjogren's syndrome-induced Qi-Yin deficiency

作　　者：陈慕芝[1] 孙学斌[2] 巴哈尔古丽.力提甫 照日格图[1] 孙红艳[3] 段沐含 CHEN Muzhi;SUN Xuebin;Bahaerguli Litifu;ZHAO RI Getu;SUN Hongyan;DUAN Muhan(Department of Rheumatism, the Affiliated Traditional Chinese Medicine Hospital,Xinjiang Medical University, Urumqi 830000, China;Orthopedic Center,the First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China;Xinjiang Medical University, Urumqi 830011, China)

机构地区：[1]新疆医科大学附属中医医院风湿科,乌鲁木齐830000 [2]新疆医科大学第一附属医院骨科中心,乌鲁木齐830054 [3]新疆医科大学,乌鲁木齐830011

出　　处：《新疆医科大学学报》2018年第1期113-116,共4页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区自然科学基金青年科学基金项目(2015211C154)

摘　　要：目的探讨原发性干燥综合征(pSS)患者外周血单个核细胞(PBMC)中miR-155的表达水平,并分析pSS气阴两虚证与非气阴两虚证在临床各项指标之间的差异。方法选取在新疆医科大学附属中医医院风湿科就诊的pSS患者60例(pSS组),其中气阴两虚证30例和非气阴两虚证30例;选择同期在本院体检的健康人30例为对照(对照组)。采用实时荧光定量(PCR)法检测2组PBMC中miR-155的相对表达量,并且分析pSS气阴两虚证与非气阴两虚证在临床各项指标之间的差异。结果 pSS组PBMC中miR-155的相对表达水平(0.910±0.153)较健康对照组(1.024±0.222)明显下调,差异有统计学意义(t=2.519,P=0.016)。pSS气阴两虚证组患者PBMC中miR-155的相对表达水平低于pSS非气阴两虚证组,差异有统计学意义(P<0.05);pSS气阴两虚证组病程、血沉(ESR)、血小板(PLT)、免疫球蛋白(Ig A、Ig G)及补体C3、C4与非气阴两虚证组比较差异无统计学意义(P>0.05)。结论 miR-155在pSS患者PBMC中显著低表达,pSS气阴两虚证患者病程长、疾病活动度高,病情较重。Objective To study the expression level of miR-155in peripheral blood mononuclear cell(PBMC)in patients with primary Sjogren′s syndrome(pSS)and to analyze the differences in multiple clinical indicators between pSS with and without Qi-Yin deficiency.Methods Sixty patients with pSS from traditional Chinese medicine institute in Xinjiang Uygur Autonomous Region were selected:half with Qi-Yin deficiency and half without Qi-Yin deficiency.Another thirty healthy individuals were served as controls.Using real-time fluorescence quantitative polymerase chain reaction method,the expression level of miR-155in PBMC between groups were determined and the multiple clinical indicators were analyzed between pSS with and without Qi-Yin deficiency.Results The expression level of miR-155was down-regulated in pSS groups(0.910±0.153)when compared to control groups(1.024±0.222).Their differences were significant(t=2.519,P=0.016).The expression level of miR-155was also down-regulated in pSS with Qi-Yin deficiency when compared to that in pSS without Qi-Yin deficiency.Their differences were also significant(P<0.05).There were no differences(P>0.05)in multiple clinical indicators,including pathology,blood sedimentation,PLT,immunoglobulin IgA,IgG and complement C3,C4between pSS with and without Qi-Yin deficiency.Conclusion The miR-155in PMBC is significantly less expressed for pSS patients.Especially,pSS patients with Qi-Yin deficiency have a long disease course,high disease activity,and more serious conditions.

关 键 词：原发性干燥综合征 气阴两虚证 MIR-155 

分 类 号：R593.2[医药卫生—内科学]