利用SNP芯片和BSA分析规模化定位小麦抗白粉病基因  

Large Scale Detection of Powdery Mildew Resistance Genes in Wheat via SNP and Bulked Segregate Analysis

作　　者：吴秋红 陈永兴 李丹[2] 王振忠[3] 张艳[2] 袁成国 王西成[5] 赵虹[5] 曹廷杰[5] 刘志勇 WU Qiu-Hong;CHEN Yong-Xing;LI Dan;WANG Zhen-Zhong;ZHANG Yan;YUAN Cheng-Guo;WANG Xi-Cheng;ZHAO Hong;CAO Ting-Jie;LIU Zhi-Yong(Institute of Genetics and Developmental Biology,Chinese Academy of Science,Beijing100101,China;China Agricultural University,Beijing100193,China;China’s Rural Technology Development Center,Beijing100045,China;Gaoyi Seeds Farm,Gaoyi051330,Hebei,China;Wheat Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou450002,Henan,China)

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]中国农业大学,北京100193 [3]中国农村技术开发中心,北京100045 [4]河北省高邑县原种场,河北高邑051330 [5]河南省农业科学院小麦研究所,河南郑州450002

出　　处：《作物学报》2018年第1期1-14,共14页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0101004)资助~~

摘　　要：规模化定位小麦品种携带的抗白粉病基因对于抗病性种质创新和新品种选育具有重要的意义。本研究采用Illumina Infinium i Select 90k SNP芯片结合集群分离分析法(bulked segregate analysis,BSA)对36个河南省小麦新品系携带的抗白粉病基因进行了定位。SNP芯片检测表明,在24个小麦品系构建的抗、感池DNA间可检测到一个明显富集的SNP峰,表明其可能携带单一主效抗白粉病基因;在其他12个小麦品系构建的抗、感池DNA间可检测到多个SNP峰,推测其可能含多个抗白粉病基因。有26个小麦品系在2AL染色体上检测到的SNP数目最多,推测其携带位于2AL染色体上的Pm4b抗白粉病基因。开发出与2AL染色体上抗白粉病基因紧密连锁的分子标记Xwggc116,可用于这些小麦品系中抗白粉病基因的分子检测。研究结果表明,高通量SNP分析技术平台可以用来规模化定位小麦品种中的抗白粉病基因。明确了河南省抗白粉病小麦品系中携带Pm2、Pm4b、Pm21和新1BL/1RS易位等有限的抗白粉病基因,抗病基因资源非常狭窄,亟需引进新的多样化抗病基因资源,拓宽遗传基础,培育抗病小麦新品种。Large-scale detection of powdery mildew resistance genes is necessary for wheat germplasm innovation and breeding,especially via marker assisted selection.Illumina90k iSelect SNP chip and Bulked Segregate Analysis(BSA)were applied to identify powdery mildew resistance gene in36wheat varieties(lines)from Henan province.SNP genotyping between36resistantbulks and36susceptible bulks revealed that single polymorphic SNP peaks were identified between24of the36bulk pairs,indicating single powdery mildew resistance gene may present in the24varieties(lines).Multiple polymorphic SNP peaks were found between other12resistant and susceptible bulks,indicating more than one powdery mildew resistance gene might be in these varieties(lines).Among the36bulk pairs,26showed the largest number of SNP enriched on chromosome2AL,indicating the powdery mildew resistance genes,most likely on Pm4locus,were in these26varieties(lines).A new marker Xwggc116was developed and proved to be effective for detecting the powdery mildew resistance gene on2AL.Overall,the combination of BSA and high-throughput SNP genotyping platform is highly effective for large scale powdery mildew resistance gene detection in wheat germplasm.There are a limited number of powdery mildew resistance genes(Pm2,Pm4,Pm21,and new1BL/1RS translocation)in wheat varieties(lines)of Henan province,indicating very narrow genetic diversity of the powdery mildew resistance genes in wheat breeding program.Exploring and utilization of new diversified disease resistance genes are urgent for breeding new varieties with disease resistance.

关 键 词：小麦品系 抗白粉病基因 BSA SNP 

分 类 号：S435.121.46[农业科学—农业昆虫与害虫防治]