^(12)C^(6+)离子束辐照糜子诱变突变群体的构建与SSR分析  

Pedigree Construction and SSR Analysis of Broomcorn Millet Mutant by ^(12)C^(6+) Ion Beam Irradiation

作　　者：刘天鹏[1] 董孔军[1] 董喜存[2] 何继红[1] 刘敏轩[3] 任瑞玉[1] 张磊[1] 杨天育[1,4] LIU Tian-Peng;DONG Kong-Jun;DONG Xi-Cun;HE Ji-Hong;LIU Min-Xuan;REN Rui-Yu;ZHANG Lei;YANG Tian-Yu(Crop Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou730070,Gansu,China;Institute of Modern Physics,Chinese Academy of Sciences,Lanzhou730000,Guansu,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing100081,China;Life Sciences and Technology College,Gansu Agricultural University,Lanzhou730070,Guansu,China)

机构地区：[1]甘肃省农业科学院作物研究所,甘肃兰州730070 [2]中国科学院近代物理研究所,甘肃兰州730000 [3]中国农业科学院作物科学研究所,北京100081 [4]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《作物学报》2018年第1期144-156,共13页Acta Agronomica Sinica

基　　金：甘肃省农业科学院中青年基金项目(2016GAAS36);国家现代农业产业技术体系建设专项(CARS-06-13.5-A9)资助~~

摘　　要：为构建物理诱变糜子突变体库,采用50、100、150、200和250 Gy剂量碳离子束(^(12)C^(6+))辐照陇糜7号和晋黍9号种子,结合混合系谱法和系统聚类分别构建2个含52和79个株系的M5寡表型突变群体。田间试验结果显示,诱变M1出苗率随剂量增大明显降低,陇糜7号M1半致死剂量为150 Gy,晋黍9号M1半致死剂量为100 Gy,且100、150 Gy诱变下M4变异最多。不同诱变剂量下M5、M6表型稳定且株高、产量性状、成株色及粒色均表现出明显遗传差异。从2个M6群体中分别选择9和11个代表性株系,利用多态性SSR引物进行分子验证,与亲本相比,6对SSR引物在陇糜7号的9个株系中位点变异基因型数为1~2,在晋黍9号的11个株系中位点变异基因型数为1~4,突变群体存在丰富的遗传多样性。To construct mutant library of physical mutation in broomcorn millet,applied five doses of12C6+ion beam at50,100,150,200,and250Gy to irradiate seed of Longmi7and Jinshu9,and constructed two populations(M5)consisting of52and79lines respectively with mixed pedigree method and system cluster.The field text showed that the emergence rate of M1decreased significantly with increasing12C6+dose,half lethal dose for M1from Longmi7and Jinshu9were150Gy and100Gy.M4at100,150Gy produce a most abundant mutants.M5and M6Phenotypes were more stable than M4,and plant height,yield traits,plant color and seed color showed significant difference.Nine M6lines from Longmi7and11M6lines from Jinshu9were detected with six pairs of SSR primer,compared with the parents,loci variant genotype number were1–2and1–4,respectively,showing abundant genetic diversity in mutant populations.

关 键 词：碳(12C6+)离子 物理诱变 突变体 谱系 SSR标记 

分 类 号：S516[农业科学—作物学]