青蒿琥酯调节Claudin-5基因表达影响胶质瘤细胞力学特性的研究  

Effects of Artesunate on gene Claudin-5 expression which could influent cell mechanical properties in Glioma

作　　者：连世忠[1] 冯靖 苗旺[1] 郭庚[1] 王宏勤 胡晓玲[2] 宋彬[3] 徐勇明[1] 徐茗 黄训 师如意[2] Lian Shizhong;Feng Jing;Miao Wang;Guo Geng;Wang Hongqin;Hu Xiaoling;Song Bin;Xu Yongming;Xu Ming;Huang Xun;Shi Ruyi(Department of Neurosurgery, First Hospital of Shanxi medical University, Taiyuan 030001, China;Basic Medical School, Shanxi Medical University, Taiyuan 030001, China;Department of Oncology, First Hospital of Shanxi Medical University, Taiyuan 030001;First Clinical Medical College, Shanxi Medical University, Taiyuan 030001, China;Department of Orthopedics, the First Affiliated Hospital of Ji’nan University, Guangzhou 510630, China)

机构地区：[1]山西医科大学第一医院神经外科,太原030001 [2]山西医科大学基础医学院,太原030001 [3]山西医科大学第一医院肿瘤科,太原030001 [4]山西医科大学第一临床医学院,太原030001 [5]暨南大学附属第一医院骨科,广州510630

出　　处：《中华神经创伤外科电子杂志》2017年第6期360-364,共5页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：山西省基础研究计划(2015011095)

摘　　要：目的检测青蒿琥酯(Art)对胶质瘤SHG44细胞中Claudin-5基因表达及细胞力学特性的影响。方法利用多聚酶链式反应法、原子力显微镜检测法、trans-well法分别检测Art对Claudin-5基因表达、SHG44细胞力学特性及细胞迁移能力等方面的影响。结果 Art可显著上调细胞间紧密连接蛋白Claudin-5基因表达;同时可以明显改变SHG44细胞力学特性:30 mg/l Art处理后的细胞粘附力显著升高(2600±400)p N vs(3800±300)p N,细胞弹力显著下降(26±6)MPa vs(4.7±1.6)MPa,细胞表面粗糙度大幅上升(0.150±0.013)μm vs(0.330±0.022)μm,可有效抑制SHG44细胞的迁移。结论实验结果表明Art可以通过影响Claudin-5基因表达,从而改变SHG44细胞力学特性进而发挥其抗癌作用,是潜在的胶质瘤治疗新药。Objective To observe the effects of Artesunate(Art)on expression of gene Claudin-5in Glioma and cell mechaniacal properties by different biological detecting methods.Methods After treated by Art,the expression of gene Claudin-5in SHG44had been detected by polymerase chain reaction method;cell migration had been detected by trans-well method.And we used atomic force microscope to observe the change of cell mechanical properties.Results The Art could upregulate the expression of gene Claudin-5,which is the intercellular tight junction protein,leading to change of the mechanical properties of SHG44cells to inhibit SHG44cell migration effectively.The cell adhesive force increased significantly after30mg/l Art treatment(2600±400)pN vs(3800±300)pN.And the cell elasticity decreased significantly after30mg/l Art treatment(26±6)MPa vs(4.7±1.6)MPa.The membrane surface roughness of SHG44increased significantly(0.15±0.013)μm vs(0.33±0.022)μm after30mg/l Art treatment.Conclusion The Art,which could be a potential anti-tumor drug,could affect the biological characteristics of SHG44through regulating the expression of gene Claudin-5to resist Glioma.

关 键 词：青蒿琥酯 胶质瘤 Claudin-5基因 细胞力学特性 

分 类 号：R285[医药卫生—中药学]