金黄色葡萄球菌L型对宫颈癌细胞生长的影响  

Effect of staphylococcus aureus L-form on the growth of cervical cancer cells

作　　者：胡小冬[1] 崔洁[2] 唐素兰[2] 管俊昌[3] HU Xiaodong;CUI Jie;ANG Sulan;GUAN Junchang(Department of Histology and Embryology,Bengbu Anhui 233030,China;Comprehensive Laboratory of Microscopic Morphology,Bengbu Anhui 233030,China;Department of Microbiology,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院组织胚胎学教研室,安徽蚌埠233030 [2]蚌埠医学院显微形态学综合实验室,安徽蚌埠233030 [3]蚌埠医学院微生物学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2017年第11期1436-1438,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院科技发展基金面上项目(Bykf13A23)

摘　　要：目的:研究金黄色葡萄球菌(金葡菌)L型对体外培养的单层人宫颈癌细胞(He La)生长的影响作用。方法:用常规方法诱导金葡菌为L型后感染He La细胞,采用光学显微镜、透射电子显微镜检测金葡菌L型黏附及侵入细胞情况,并采用改良的MTT比色法检测细胞增殖能力。结果:光镜、电镜检查显示金葡菌L型能够黏附并且进入He La细胞;改良的MTT法检测表明金葡菌L型能够促进He La细胞增殖(P<0.01)。结论:金葡菌L型能够感染并促进He La细胞增殖生长,揭示金葡菌L型可能与宫颈癌的发生有关。Objective:To observe the effects of Staphylococcus aureus L-form on the growth of human cervical cancer cells(HeLa)in vitro.Methods:The HeLa cells were infected with Staphylococcus aureus L-form by routine method.The adhesion and invasion of Staphylococcus aureus L-form were detected using light and electron microscopy,and the proliferation of HeLa cells was detected using improved MTT assay.Results:The results of light and electron microscopy L-form showed that the Staphylococcus aureus L-form could adhere and invade into HeLa cells.The result of improved MTT method showed that Staphylococcus aureus L-form could promote the proliferation of HeLa cells.Conclusions:Staphylococcus aureus L-form can infect and promote the growth of HeLa cells,which reveals that Staphylococcus aureus L-form may be one of inducting factor of cervical cancer.

关 键 词：金黄色葡萄球菌 细菌L型 宫颈癌细胞 

分 类 号：R378.11[医药卫生—病原生物学]