拟南芥HECTE3s家族基因表达模式和功能的初步研究  

Expression Profile and Function of HECT E3s in Arabidopsis thaliana

作　　者：兰蔚 邱士优 任育军[1] 缪颖[1] LAN Wei;QIU Shiyou;REN Yujun;MIAO Ying(Center for Molecular Cell and Systems Biology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University,Fuzhou 350002, China)

机构地区：[1]分子细胞与系统生物学中心,福建省海峡植物应用系统生物学重点实验室,福建农林大学生命科学学院,福州350002

出　　处：《西北植物学报》2017年第11期2112-2119,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31470383)

摘　　要：利用GUS组织化学染色法,研究由拟南芥泛素蛋白连接酶HECT家族基因(UPLs)启动子起始的β-葡萄糖苷酸酶报告基因(GUS)的表达模式。结果表明:UPLs家族中6个成员的启动子在拟南芥植株的不同组织和不同时期均有所表达,且在莲座叶发育的早期和衰老期表现出较高活性。进一步观察upl3和upl4突变体发现,upl3和upl4突变体除了upl3有较明显的叶型变化,两者均表现为延迟衰老现象;且突变体中衰老相关基因的表达水平不同于野生型。研究推测UPL3和UPL4可能参与了叶片生长发育过程的调控,同时还参与调控植株衰老进程。In this research,we use GUS histochemical staining method to analyze the expression pattern of GUS gene of Pro UPLs∶GUS transgenic plant,which initiate the GUS report gene by the promoter of HECT E3s genes(UPLs).The results of these six HECT E3s genes at different development stages and tissues show different expression patterns,but all of them have high activity at the early stage and senescence stage of leaf development.Interestingly,we find that the leaf shape of upl3mutant show significant difference and both upl3and upl4mutant delay senescence and have different expression levels of senescence-associated genes compared to wild type.Thus,we speculate that both UPL3and UPL4are likely to regulate the leaf development and the progress of plant senescence.

关 键 词：拟南芥 HECT泛素蛋白连接酶家族 GUS染色 表达模式 

分 类 号：Q786[生物学—分子生物学] Q789