小麦PPO活性基因等位变异的区域分布研究  

Geographic Distribution of Allelic Variation of Polyphenol Oxidase (PPO) Gene in Wheat Cultivars from Different Ecological Regions

作　　者：王黎明[1,2] 高华利[1,3] 李永霞 董普辉 王春平[1] 李兴锋[2] WANG Liming;GAO Huali;LI Yongxia;DONG Puhui;WANG Chunping;LI Xingfeng(Agronomy College, Henan University of Science and Technology, Luoyang, Henan 471003,China;State key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Taian, Shandong 271018, China;Huangfanqu Farm in Henan Province, Zhoukou, Henan 466000, China)

机构地区：[1]河南科技大学农学院,河南洛阳471003 [2]山东农业大学农学院,作物生物学国家重点实验室,山东泰安271018 [3]河南省黄泛区农场,河南周口466000

出　　处：《西北植物学报》2017年第12期2370-2377,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(U1304318);山东农业大学作物生物学国家重点实验室开放基金(2008KF01);河南省自然科学基金(162300410077);河南省国际合作项目(172102410052)

摘　　要：为了解中国不同生态区小麦种质资源籽粒多酚氧化酶(PPO)活性基因的等位变异的差异与分布,利用小麦PPO活性基因的功能标记PPO16、PPO29与PPO18,检测了来自中国7个不同生态麦区的379份小麦种质资源的等位变异和分布差异。结果表明:(1)在2AL染色体该基因位点有2种等位变异类型:Ppo-A1a(高PPO)和Ppo-A1b(低PPO),其频率分别为51.5%和48.5%。(2)在2DL染色体该基因位点有3种等位变异类型:Ppo-D1a(低PPO)、Ppo-D1b(高PPO)和Ppo-D1ab(中间型),其频率分别为57.8%、32.5%和9.8%。(3)该基因在2AL和2DL染色体上的位点变异有6种不同类型的组合:Ppo-A1a/D1a(中间型)、Ppo-A1a/D1b(高PPO)、Ppo-A1a/Ppo-D1ab(中间型)、Ppo-A1b/D1a(低PPO)、Ppo-A1b/D1b(中间型)和Ppo-A1b/Ppo-D1ab(中间型),其中,与低PPO活性相关的基因型组合Ppo-A1b/D1a的频率为25.6%。(4)小麦PPO活性基因不同变异类型在各生态区的分布存在明显差异,基因型Ppo-A1b在北部冬麦区和西南冬麦区的比例较大,基因型Ppo-D1a在黄淮冬麦区和北部冬麦区的比例较大,基因型组合Ppo-A1b/D1a在北部冬麦区的比例较大。研究认为,结合采用分子标记辅助选择(MAS),有利于小麦籽粒外观品质的遗传改良和新品种选育。Study on distribution of PPO gene activity in different wheat ecological region cultivars would benefit screening elite germplasms for improving wheat quality.A total of379wheat cultivars were genotyped by the functional markers PPO16,PPO29and PPO18.The results indicated that:(1)There were two types of PPO genes on2AL chromosomes:Ppo A1a(high PPO)and Ppo A1b(low PPO),and their frequency were51.5%and48.5%,respectively.(2)there were three types of PPO genes on2DL chromosomes:Ppo D1a(low PPO),Ppo D1b(high PPO)and Ppo D1ab(intermediate type),and their frequency were57.8%,32.5%and9.8%,respectively.(3)There were six different gene combinations on the chromosome of2AL and2DL:Ppo A1a/D1a(intermediate type),Ppo A1a/D1b(high PPO),Ppo A1a/Ppo D1ab(intermediate type),Ppo A1b/D1a(low PPO),Ppo A1b/D1b(intermediate type)and Ppo A1b/Ppo D1ab(intermediate type).The frequency of Ppo A1b/D1a(low PPO)was25.6%.(4)The distribution frequency about different variation types of wheat PPO gene in various ecological regions existed obvious differences:the proportion of the genotype of Ppo A1b was larger in northern winter wheat region and south western winter wheat region,and,the proportion of the genotype Ppo D1a was larger in Yellow and Huai facultative winter wheat area and northern winter wheat region.The proportion of genotype combination Ppo A1b/D1a was larger in northern winter wheat region.Therefore,molecular marker assisted selection(MAS)method could be considered for genetic improvement of appearance quality of wheat grain,and through introducing fine germplasm from the northern winter wheat region.

关 键 词：普通小麦 生态区 PPO 功能标记 MAS 

分 类 号：Q789[生物学—分子生物学] S512.1[农业科学—作物学]