贵州汉族人群15个STR基因座遗传多态性研究  

Study on genetic polymorphism of 15 STR loci in Han nationality from Guizhou

作　　者：胡锡阶[1,2] 刘祖林[1,2] 余丽梅[1,2] 万卫红[1,2] Hu Xijie;Liu Zulin;Yu Limei;Wan Weihong(Key laboratory of Cell Engineering of Guizhou Province,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563099,China;Department of Forensic Judicial Authentication Center,Affiliated Hospital of Zunyi Medical University,Zunyi Guizhou 563099,China)

机构地区：[1]遵义医学院附属医院贵州省细胞工程重重点实验室,贵州遵义563099 [2]遵义医学院附属医院司法鉴定中心,贵州遵义563099

出　　处：《遵义医学院学报》2017年第6期599-602,608,共5页Journal of Zunyi Medical University

基　　金：贵州省生物治疗人才基地定向招标课题

摘　　要：目的对贵州汉族人群在Identifiler体系中的15个短串连重复序列(STR)基因座进行遗传多态性调查。方法采用荧光标记STR-PCR复合扩增、毛细管电泳基因分型技术,对1 000名贵州汉族人群无关个体进行15个STR基因座的基因分型,计算其等位基因频率、杂合度(H)、匹配概率(PM)、个人识别力(DP)、非父排除率(PE)、多态信息总量(PIC)、父权指数(PI)等多态性参数,并进行统计学分析。结果在贵州汉族人群1 000名无关个体中共检出178个等位基因,其频率分布为0.000 5~0.531 5。15个STR基因座的基因型分布进行χ~2检验符合Hardy-Weinberg平衡定律(P>0.05)。杂合度为0.603 0~0.880 0;个人识别力为0.786 1~0.966 6,累积DP>0.999 999 999;匹配概率为0.033 4~0.213 9,累积PM=1.198×10^(-17);三联体非父排除率为0.294 5~0.754 8,累积CPE=0.999 998 925;二联体非父排除率为0.195 9~0.571 6,累积CPE=0.999 760 600;多态信息含量为0.545 1~0.849 2,累积PIC=0.999 999 999;亲权指数为1.2 594~4.1 667。结论贵州汉族人群检测的15个STR基因座具有高度多态性,是较理想的遗传标记,统计的等位基因频率等基础数据可为本地区的个人识别、三联体亲子鉴定及群体遗传学研究提供依据,但在二联体亲子鉴定中运用存在一定风险。Objective To investigate the genetic polymorphism of15STR loci with Identifiler kit in Han nationality in Guizhou province.MethodsThe technique of fluorescent dye labeling multiplex STR-PCR,capillary electrophoresis and genotyping techniques were adopted in genotyping1000unrelated individuals collected from Han nationality in Guizhou.The allele frequency of15STR loci,the heterzygosity(H),the discrimination power(DP),the probability of matching(PM),the power of exclusion(PE),the polymorphism information content(PIC),and the paternity index(PI)were calculated.ResultsAmong the Han population samples of1000unrelated individuals,the total of178alleles in15STR loci with their frequencies of0.0005~0.5315were detected.The genotypic distributions meet Hardy-Weinberge equilibrium byχ2testing(P>0.05).Statistical result showed that the heterzygosity(H)were ranged from0.6030to0.8800,the power of discrimination(DP)were0.7861~0.9666,the combined DP>0.999999999,the probability of matching(PM)were0.0334~0.2139,the combined PM were1.198×10-17,the power of exclusion in Trios(PE)were0.2945~0.7548,the combined PE were0.999998925,the power of exclusion in Duos were0.1959~0.5716,the combined PE were0.999760600,the polymorphism information content(PIC)were0.5451~0.8492,the combined PIC=0.999999999,the paternity index(PI)were1.2594~4.1667.ConclusionThe15STR loci have higher polymorphism and are satisfactory genetic markers.The data on the allele frequencies of these15STR loci can be used in individual identification,parentage testing of trios and other population genetic researches for the Guizhou Han nationality,whereas a certain risk could be arisen in parentage testing of duos.

关 键 词：IDENTIFIER 贵州汉族人群 短串联重复序列 遗传多态性 

分 类 号：D919.4[医药卫生—法医学]