卵母细胞滑面内质网聚集对MII卵母细胞有效胚胎形成率的影响  

Effect of oocytes with smooth endoplasmic reticulum aggregation on oocyte-to-embryo rate

作　　者：潘家坪 阮井玲 黄媚媛 王羽 梁珊珊 滕晓明 吴海霞 PAN Jia-ping;RUAN Jing-ling;HUANG Mei-yuan;WANG Yu;LIANG Shan-shan;TENG Xiao-ming;WU Hai-xia(Shanghai First Maternity & Infant Hospital,Tong Ji University,Shanghai 201202)

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海201202

出　　处：《生殖医学杂志》2018年第1期16-20,共5页Journal of Reproductive Medicine

基　　金：上海市卫计委课题(201440005);卫生计生委公益项目(201402004)

摘　　要：目的探讨卵母细胞滑面内质网聚集(SERa)对卵母细胞利用率的影响。方法回顾性分析2013年1月至2014年12月的250周期ICSI治疗患者,根据卵母细胞有无SERa分为两组:SERa+组(至少有1个卵母细胞SERa阳性,A组)50周期;SERa-组(所有卵母细胞均无SERa,B组)200周期。分析两组患者的临床和实验室指标,包括受精率、异常受精率、卵裂率、有效胚胎形成率及卵母细胞利用率。结果与B组相比,A组卵裂率、有效胚胎形成率及卵母细胞利用率显著降低(P<0.05),而异常受精率显著增加(P<0.05);A组中的SERa-卵母细胞与B组卵母细胞相比,卵裂率、有效胚胎形成率及卵母细胞利用率显著降低(P<0.05),异常受精率也显著增加(P<0.05);A组中SERa+卵母细胞与SERa-卵母细胞相比,异常受精率显著增加(P<0.05),卵裂率、有效胚胎形成率及卵母细胞利用率无显著性差异(P>0.05)。结论卵母细胞胞浆内滑面内质网聚集增加异常受精率,降低卵母细胞利用率。To study the effect of smooth endoplasmic reticulum aggregation(SERa)in oocytes on oocyte to embryo rate Methods:The data of250cycles of ICSI treated patients from January2013to December2014were retrospectively analyzed According to whether oocytes had SERa,the cycles were divided into two groups:at least one oocyte with SERa positive in SERa+group(group A,50cycle)and all oocytes without SERa in SERa-group(group B,200cycle)The fertilization rate,abnormal fertilization rate,cleavage rate,available embryos rate and oocyte to embryo rate were compared between the two groups Results:The significantly lower cleavage rate,available embryos rate,oocyte to embryo rate and significantly higher multiple pronucleus fertilization rate were observed in the SERa+cycles as compared to SERa-cycles(P<0.05)SERa+cycles had significantly lower cleavage rate,available embryos rate&oocyte to embryo rate,and significantly higher multiple pronucleus fertilization rate(P<0.05)compared with SERa-oocyes Among SERa+cycles,SERa+oocytes had higher multiple pronucleus fertilization rate compared to SERa-oocytes(P<0.05),while there was no significant difference in cleavage rate,available embryos rate and oocyte to embryo rate between SERa+oocytes and SERa-oocytes(P>0.05)Conclusions:Oocyte cytoplasmic smooth endoplasmic reticulum aggregation increases abnormal fertilization rate and reduces oocyt to embryo rate

关 键 词：卵母细胞 ICSI 滑面内质网 卵母细胞利用率 

分 类 号：R714.8[医药卫生—妇产科学]