外源多胺促进的霍山石斛类原球茎芽发生与H_2O_2代谢的关系  

Relationship between Enhanced Shoot Conversion and H_2O_2-metabolism of Protocorm-like Bodies of Dendrobium huoshanense by Exogenous Polyamines

作　　者：杨光 罗建平 王瑛[2] YANG Guai;LUO Jianping;WANG Ying(School of Food Science and Engineering, Hefei University of Technology, Anhui, Hefei230009;College of Life Science, Hebei University, Baoding,Hebei 071002)

机构地区：[1]合肥工业大学食品科学与工程学院,安徽合肥230009 [2]河北大学生命科学学院,河北保定071002

出　　处：《安徽农业科学》2018年第1期1-4,23,共5页Journal of Anhui Agricultural Sciences

基　　金：河北大学自然科学研究计划项目(2010-195)

摘　　要：[目的]研究外源多胺促进霍山石斛类原球茎芽的发生与过氧化氢(H_2O_2)代谢酶活性的关系。[方法]主要测定了外源多胺处理后H_2O_2的动态变化和代谢酶的活性。[结果]外源多胺在有效促进芽发生的同时也能显著提高培养物中H_2O_2的含量,2.0 mmol/L亚精胺(Spd)或腐胺(Put)处理5 d的培养物中H_2O_2含量分别比对照组高175.6%和180.6%,添加H_2O_2淬灭剂二甲基硫脲(DMTU)可抑制类原球茎芽的发生,补充Spd或Put可部分解除DMTU的抑制作用。在外源多胺处理前期和中期,虽然超氧化物歧化酶(SOD)和过氧化物酶(POD)活性无明显改变,但合成H_2O_2的多胺氧化酶(PAO)活性和分解H_2O_2的抗坏血酸过氧化物酶(APX)与过氧化氢酶(CAT)活性明显增加。[结论]外源多胺作用的霍山石斛类原球茎芽的发生与H_2O_2积累和分解代谢有关。[Objective]The research aimed to study the relationship between enhanced conversion of protocorm-like bodies(PLBs)of Dendrobiumhuoshanense to shoots by exogenous polyamines(PA s)and H202-metabolizing enzyme activities.[Method]W e mainly measured the dynamicchanges of H202and the activities of metabolizing enzyme after exogenous polyamines treatment.[Result]Exogenous addition of PAs not only promoted the conversion frequency of PLBs to shoots but also elevated the levels of H202,compared with the control,the H202levels in PAs groups increased by175.6%(Spd)and180.6%(Put)on the5th day after being treated using2.0mmol/L Spd or Put for5days.Dimethylthiourea(DMTU),the scavenger of H202,inhibited conversion of PLBs to shoots and the effect could be offset partly by exogenous PAs.Although the activities of superoxide dismutase(SO D)and peroxidase(POD)did not change significantly in the early and middle stages of exogenous polyamine treatment,the activity of polyamine oxidase(PAO)for synthesizing H202,ascorbate peroxidase(APX)for dissolving H202and catalase(CAT)were significantly increased.[Conclusion]The occurrence of protocorm buds of Dendrobium huoshanense with the action of exogenous polyamines is related to the accumulation and catabolism of H202.

关 键 词：霍山石斛 外源多胺 类原球茎 芽发生 代谢酶 

分 类 号：S567.2[农业科学—中草药栽培]