4株奶牛源左氏无绿藻的分离与鉴定  

Isolation and Identification of 4 Prototheca zopfii Sourced from Cow

作　　者：戴迪[1] 廖俊[1] 林伟东[1] 冯清[1] 黄攀 梁祥焕 王建 秦爱建[1] 金文杰[1] DAI Di;LIAO Jun;LIN Weidong(of Veterinary Medicine , Yangzhou University,Jiangsu Key Laboratory of Animal Preventive Medicine , Jiangsu Collaborative Innovation Center of Animal Epidemic Disease and Zoonosis Control and Prevention, Yangzhou, Jiangsu 225009)

机构地区：[1]扬州大学兽医学院,江苏省动物预防医学重点实验室,江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [2]扬州大学实验农牧场,江苏扬州225009 [3]武进区动物疫病预防控制中心,江苏常州213000

出　　处：《安徽农业科学》2018年第1期78-79,86,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省“青蓝工程”;江苏省农业科技自主创新资金项目(CX(14)2088);江苏高校优势学科建设工程资助项目(PAPD);常州市科技支撑项目(CE20152006);扬州大学科创项目(x20160716);江苏省人兽共患病学重点实验室开放课题(R1607)

摘　　要：无绿藻是一种普遍存在的微藻,属于绿球藻,可引起人类和动物广泛地感染,其中的左氏无绿藻与一种严重的牛乳腺炎有关。该研究采集江苏部分地区患乳房炎奶牛奶样,用绵羊血琼脂培养、分离,用革兰氏染色镜检及PCR方法确定得到4株左氏无绿藻(P.zopfii)。这是首次在江苏地区发现奶牛源左氏无绿藻。Prototheca is a widespread microalgae,which belongs to Chlorococcumsp.People and animal can commomly infect prototheca.Among of them,Prototheca zopfii was related to severe mastitis of cow.Milk samples of cow having mastitis were collected from part of Jiangsu.4P.zopfii were identified by gram stain microscopy and PCR method using sheep blood agar to cultur and isolate them.P.zopfii sourced from cowed was first discovered in Jiangsu area.

关 键 词：奶牛乳房炎 左氏无绿藻 PCR鉴定 

分 类 号：S852.66[农业科学—基础兽医学]