四爿藻培养模式的筛选及其培养基的优化  

Screening of Cultivation Mode for Tetraselmis chui and Optimizing Its Culture Medium

作　　者：符新欢 王珺[1,2,3] 王永强[1,2,3] 梁业松 陈国华[1,2] 詹景福 FU Xinhuan;WANG Jun;WANG Yoiqiang(Ocean College, Hainan University, Haikou, Hainan 570228;Key Laboratory of Tropic Biological Resources, Ministry of Education, Hainan University, Haikou, Hainan 570228;Experimental Teaching Center of Marine Biology, Hainan University, Haikou, Hainan 570228)

机构地区：[1]海南大学海洋学院,海南海口570228 [2]海南大学,热带生物资源教育部重点实验室,海南海口570228 [3]海南大学海洋生物实验教学中心,海南海口570228

出　　处：《安徽农业科学》2018年第1期80-83,101,共5页Journal of Anhui Agricultural Sciences

基　　金：海南省科技项目(ZDYF2016087);国家星火计划(2015GA800003)

摘　　要：[目的]为了提高热带四爿藻的生长速率。[方法]以"宁波大学3#微藻培养基"为基础,分别添加有机碳(葡萄糖和乙酸钠)对四爿藻进行自养、兼养及异养培养,筛选出促进四爿藻快速生长的培养模式,并通过单因子及正交试验,优化其培养基配方。然后用优化培养基与"宁波大学3#微藻培养基"对比培养四爿藻。[结果]乙酸钠是四爿藻兼养培养的适宜有机碳,其最佳的乙酸钠浓度为6 g/L。获得了四爿藻的兼养培养基为:6 g/L CH3COONa、50 mg/L NH2CONH2-N、2 mg/L Ca(H2PO4)2-P、1 mg/L Fe SO4-Fe、1 mg/L Vitamin B1、0.000 5 mg/L Vitamin B12和0.5 mg/L Vitamin H。优化兼养培养基与"宁波大学3#微藻培养基"对比培养四爿藻的结果表明,培养第7天,兼养培养收获的藻细胞密度、干重分别达到3.84×106cells/m L及1.14 g/L,分别是"宁波大学3#微藻培养基"的2.83倍、3.12倍。[结论]四爿藻进行兼养培养可获得高密度培养。[Objective]To improve the growth rate of Tetraselmis chui.[Method]Based on“Ningbo University#3medium”,organic carbon(C6H1206and CH3C00Na)was added for the autotrophic culture,mixed culture and heterotrophic culture,respectively,and the mixotrophy medium of T.chui was further optimized by single factor test and orthogonal test.And then,the results of mixotrophic and autotrophic cultures were compared.[Result]CH3C00Na was the better organic carbon applicable for the mixotrophic culture on T.chui.The optimization of mixotrophy medium was6g/L CH3C00N a,50mg/L NH2CONH2-N,2mg/L Ca(H2P04)2-P,1mg/L FeS04-Fe,1mg/L Vitamin Bj,0.0005mg/L Vitamin B12and0.5mg/L Vitamin H.The cell density and dry weight of the‘‘mixotrophy medium”was up to3.84x106cells/mL and1.14g/L after culturing seventh day,respectively,being2.83/3.12times higher than the cell density and dry weight of“Ningbo University#3”medium.[Conclusion]T.chui can be cultured with high cell density through mixotrophic culturing.

关 键 词：四爿藻 兼养 培养基 生长 

分 类 号：Q949.2[生物学—植物学]