抗虫转基因欧洲黑杨鲜叶对小鼠的急性毒性  

Acute Toxicity of Fresh Leaves of Insect Resistant Transgenic Populus nigra. to Mice

作　　者：刘海涛 汪承润 刘玲 郑勇奇[2] 张川红[2] LIU Haitao;WANG Chengrun;LIU Ling(Bioengineering Institute, Huainan Normal University, Huainan, Anhui 232038;Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091)

机构地区：[1]淮南师范学院生物工程学院,安徽淮南232038 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《安徽农业科学》2018年第1期94-95,136,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽省自然科学基金项目(1608085QC50);安徽省2016年高校优秀中青年骨干人才国内外访学研修重点项目(gxfx ZD2016202);淮南师范学院2015年度科学研究重点项目(2015xj06zd)

摘　　要：[目的]为了研究抗虫转基因欧洲黑杨对小鼠的急性毒性,为其生物安全性评估提供依据。[方法]以转基因欧洲黑杨鲜叶和非转基因欧洲黑杨鲜叶作为受试物,采用限量法测定小鼠的急性经口毒性反应。[结果]灌胃后7 d时间内所有处理组无小鼠死亡,一般状况观察和大体解剖也未见明显异常和毒性反应,小鼠LD50>10.0 g/kg体重,表明其属于"实际无毒"级别。组织病理学切片检查发现转基因灌胃组中有一雌性小鼠肺部出现间质性炎症及一雄性小鼠肝部出现局部胞浆淡染、细胞轮廓不清及空泡样变的现象,具体原因需进一步深入研究。[结论]转基因欧洲黑杨和非转基因欧洲黑杨鲜叶对小鼠均无明显急性毒性作用。[Objective]Acute toxicity of fresh leaves of Populus nigra to mice were studied to evaluate the biological safety.[Method]Taking transgenic Populus nigra and non-transgenic Populus nigra as test substance,unintended effects of insect-resistant transgenic Populus nigra was studied.[Result]There was not any death of mice after treating for7days,and no obvious acute effect in general condition and general dissecting.The half lethal dose(LD50)was greater than10.0g/kg?bw,result showed that it was classified as no toxicity.Interstitial inflammation of lung in a female mice and local pale cytoplasm,indistinct cytoplasmic borders and vacuolation of liver in a male mice have been found in the transgenic Populus nigra group by using histopathology histopathologic biopsy examine.Specific reasons still need further study.[Conclusion]It suggested that there was no obvious acute toxicity effect of transgenic P opulus nigra and non-transgenic P o pulus nigra to the test mice.

关 键 词：抗虫转基因欧洲黑杨 急性毒性 小鼠 动物试验 

分 类 号：S859.82[农业科学—临床兽医学]