哈密瓜细菌性果斑病胞外酶活性测定  

Determination of Extracellular Enzyme Activity of Hami Melon Bacterial Fruit Blotch

作　　者：毛亮 吴久赟[2] 吉艳玲 潘卫萍 张以和 王晓东 MAO Liang;WU Jiu-yun;JI Yan-ling;PAN Wei-ping;ZHANG Yi-he;WANG Xiao-dong(Xinjiang Turpan Agricultural Technology Promotion Center,Turpan, Xinjiang 838000;Turpan Research Institute of Agricultural Sciences,Xinjiang Academy of Agricultural Sciences,Turpan,Xinjiang 838000;Key Laboratory of Oasis Crop Disease Control and Prevention,Shihezi University,Shihezi,Xinjiang 832000)

机构地区：[1]新疆吐鲁番市农业技术推广中心,新疆吐鲁番838000 [2]新疆农业科学院吐鲁番农业科学研究所,新疆吐鲁番838000 [3]石河子大学绿洲农作物病害防控重点实验室,新疆石河子832000

出　　处：《黑龙江农业科学》2017年第11期30-33,共4页Heilongjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技厅基层青年科技人才资助项目(qn2015jc073);国家自然科学基金资助项目(31460485)

摘　　要：为了防治哈密瓜细菌性果斑病初期侵染,通过测定哈密瓜细菌性果斑病病菌生长曲线、病菌胞外酶、果胶酶和纤维素酶变化,掌握哈密瓜细菌性果斑病侵染初期胞外酶、果胶酶和纤维素酶活性的变化规律。结果表明:哈密瓜细菌性果斑病病菌同时产生果胶酶和纤维素酶,说明该病菌在侵染初期的致病力跟这两个酶活性有关。结合病菌生长曲线,果胶酶未呈现明显趋势,而纤维素酶随细菌的生长进入衰退期后活性呈逐渐增加趋势,在44h果胶酶和纤维素酶同时出现波峰,但后期44~72h果胶酶再没有出现大幅增长趋势,而纤维素酶呈明显上升趋势,说明病害后期的扩展纤维素酶起到了主要作用。In order to prevent the initial Hami melon bacterial fruit spot infection,through the determination of pathogen bacteria growth curve of melon bacterial fruit blotch,extracel-lular enzymes pectinase change,extracellular cellulase change,the law of extracellular enzyme pectinase and cellulose was mastered in the early stages of infection of melon bacterial fruit blotch.The results showed that pectinase and cellulase were produced by bacterial fruit blotch of melon,which indicated that pathogenicity of the pathogen was related to the activities of these two enzymes at the initial stage of infection.With the growth curve of bacteria,the pectinase did not show obvious trend,but the activity of cellulase increased gradually after the growth of bacteria entered the recession period.Both pectinase and cellulase appeared peaks at the same time in44h,but there was no significant in-crease in pectinase in the late44h to72h,cellulase showed an obvious upward trend,indicating that cellulase plays a major role in the late growth of the disease.

关 键 词：哈密瓜 细菌性果斑病 酶活性 致病性 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]