不同溶剂浸提枣树叶片叶绿素效果的研究  

Effects of Different Solutions on Chlorophyll Extraction from Jujube Leaves

作　　者：胡秉芬 黄华梨 季元祖 赵晓芳 戚建莉 张露荷 张广忠 HU Bing-fen;HUANG Hua-li;JI Yuan-zu;ZHAO Xiao-fang;QI Jian-li;ZHANG Lu-he;ZHANG Guang-zhong(Gansu Research Academy of Forestry Science and Technilogy,Lanzhou,Gansu 730020)

机构地区：[1]甘肃省林业科学研究院,甘肃兰州730020

出　　处：《黑龙江农业科学》2017年第11期48-54,共7页Heilongjiang Agricultural Sciences

基　　金：林业公益性行业科研专项资助项目(201304702)

摘　　要：为探讨枣树叶片叶绿素的最佳提取方法,以16种枣树品种为材料,用95%乙醇、乙醇丙酮混合液(1∶1)和80%丙酮分别浸提不同枣树品种叶片叶绿素,用分光光度法测定叶绿素含量,对不同浸提液的叶绿素含量进行了分析。结果表明:80%丙酮浸提枣树叶片叶绿素的效果不佳;95%乙醇和乙醇丙酮混合液(1∶1)浸提枣树叶片叶绿素的效果好,且水平相当。但丙酮对人体的健康危害较大,浸提枣树叶片叶绿素应首选95%乙醇。In order to study the optimal extraction method effect of chlorophyll from jujube leaves,chlorophyll were extracted from16kinds of jujube varieties leaves using95%ethanol,mixture of ethanol and acetone(1∶1)and80%acetone respectively,chlorophyll content was determined by spectrophotometry,the content of chlorophyll in different extracts was studied.The results showed that80%acetone was not ideal for the extraction of chlorophyll from the leaves of jujube trees;95%ethanol and mixed liquid of ethanol and ace-tone(1∶1)have a good effect on the extraction of chlorophyll from the leaves of jujube trees,and the extraction level was quite the same.But acetone was harmful to human health.95%ethanol was the first choice to extract chlorophyll from leaves of Chinese jujube.

关 键 词：枣树 叶绿素 浸提 有机溶剂 

分 类 号：S665.1[农业科学—果树学]