胶冻样芽孢杆菌发酵中试研究  

Pilot Scale Fermentation of Bacillus mucilaginous

作　　者：廖先清[1] 刘芳[1] 周荣华[1] 饶犇 陈伟[1] LIAO Xian-qing;LIU Fang;ZHOU Rong-hua;RAO Ben;CHEN Wei(Hubei Biopesticide Engineering Rescarch Center/Biopeaticide Subeenter,Hubei Scienee and Teehnology Innovation Center, Wuhan 430064,China)

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心,武汉430064

出　　处：《湖北农业科学》2017年第23期4513-4515,共3页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心资助项目(2016-620-000-001-038);湖北省技术创新专项重大项目(2016ABA103);国家重点研发计划“生物杀菌剂”项目(2017YFD0201107)

摘　　要：利用单因素筛选和正交试验,对胶冻样芽孢杆菌菌株NBF-266的发酵培养基进行了优化,得到其最佳培养基配方为甘油0.8%,淀粉0.6%,花生粕1.5%,碳酸钙0.1%。通过400 L和5 000 L发酵罐分别发酵,发酵液芽孢产量分别为18×10~8 CFU/mL和15.8×10~8 CFU/mL,发酵液经不同方式浓缩干燥,得到冻干粉和喷雾干燥粉芽孢产量分别为2 910×10~8 CFU/g和933×10~8 CFU/g。The fermentation medium for Bacillus mucilaginous NBF-266strain was optimized through single factor screening and orthogonal experiments.The composition of the optimized medium was glycerin0.8%,starch0.6%,peanut meal1.5%,calcium carbonate0.1%.Pilot production of B.mucilaginous was earned out in400L and5000L fennentor,spore yield of the fennentation broth were18xl08CFU/mL and15.8xl08CFU/mL respectively.The fennentation broth was concentrated and dried in two different ways,spore yield of the freeze-dried powder and spray diying powder were2910xl08CFU/g and933x lO8CFU/g respectively.

关 键 词：股冻样芽孢杆菌 芽孢产量 优化 中试生产 

分 类 号：Q939.96[生物学—微生物学]